基于p38 MAPK信号通路探讨固本止咳中药对慢性阻塞性肺疾病稳定期小鼠气道炎症调控作用  

Inhibition of p38MAPK signal pathway by Guben Zhike Recipe on airway inflammation in COPD mice

作　　者：刘明非 方晗语 洪政 陈宏丽 张洪春[3] LIU Mingfei;FANG Hanyu;HONG Zheng;CHEN Hongli;ZHANG Hongchun(Beijing University of Chinese Medicine,Beijing 100029,China;Department of Internal Medicine,Beijing Chaoyang TCM Hospital,Beijing 100020;China-Japan Friendship Hospital,Beijing 100029)

机构地区：[1]北京中医药大学,北京100029 [2]北京朝阳中医医院内科,北京100020 [3]中日友好医院,北京100029

出　　处：《北京中医药》2024年第6期631-635,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82074367);国家重点研发计划项目(2019YFC1712003)。

摘　　要：目的 观察固本止咳中药对慢性阻塞性肺疾病(COPD)小鼠气道炎症的调控作用。方法 将20只小鼠分为空白对照组、模型组、固本止咳中药组、地塞米松组(n=5)。除空白对照组外,其余组小鼠均采用烟熏联合鼻腔滴入脂多糖(LPS)法建立COPD动物模型。造模成功后中药组予固本止咳中药0.2 mL/d灌胃,空白对照组、模型组予等量生理盐水灌胃,地塞米松组予地塞米松注射液(1.0 mg/kg)腹腔注射,连续给药28 d,2次/d。造模期间记录小鼠一般情况,用酶联免疫吸附法检测肺组织匀浆中NO含量,用Western-blot法测定p38 MAPK、p-p38MAPK、重组人激活转录因子-2 (ATF-2)蛋白表达。结果 与模型组比较,固本止咳中药组肺组织匀浆中NO水平降低(P<0.05),ATF-2、p38MAPK蛋白表达下降(P<0.01)。结论 固本止咳中药能够改善COPD稳定期小鼠的气道炎症,机制可能与抑制p38 MAPK通路相关。Objective To observe the regulatory effect of Guben Zhike Recipe on airway inflammation in mice with chronic obstructive pulmonary disease(COPD).Methods Twenty mice were divided into a blank control group,a model group,a Guben Zhike Recipe group,and a dexamethasone group(n=5).Except for the normal group,all other mice were used to established COPD animal models by smoking combined with nasal instillation of lipopolysaccharide(LPS).After successful modeling,the traditional Chinese medicine group was given 0.2 mL/d of Guben Zhike Recipe by gavage of 0.9%physiological saline,while the blank control group and model group were given 09%physiological saline was administered by gavage,and the dexamethasone group was given dexamethasone injection(1.0mg/kg)intraperitoneal injection for 28 consecutive days,twice a day.During the modeling period,the general situations of the mice were recorded,and the NO content in lung tissue homogenate was detected using enzyme-linked immunosorbent assay.The expression of p38 MAPK,p-p398MAPK,and ATF-2 proteins was measured using Western blot.Results Compared with the model group,the NO level in the lung tissue homogenate of Guben Zhike Recipe group(for strengthening the foundation and relieving cough)was decreased(P<0.05),the expression of p38MAPK proteins decreased(P<0.01).Conclusion Guben Zhike Recipe can improve airway inflammation in stable COPD mice,and the mechanism may be related to the inhibition of the p38 MAPK pathway.

关 键 词：慢性阻塞性肺疾病 固本止咳中药 p38 MAPK通路 气道炎症 小鼠 

分 类 号：R285.5[医药卫生—中药学]