Expression changes of mi RNA-regulated genes associated with the formation of the leafy head in cabbage  

作　　者：Jorge Aleman-Baez Jose Fernando Acevedo-Zamora Johan Bucher Chengcheng Cai Roeland E.Voorrips Guusje Bonnema 

机构地区：[1]Plant Breeding,Wageningen University and Research,Wageningen,The Netherlands

出　　处：《Horticultural Plant Journal》2024年第4期1007-1019,共13页园艺学报（英文版）

基　　金：funded by the Mexican government through the Consejo Nacional de Ciencia y Tecnología (CONACYT),C.V.761325,for the PhD project of Jorge Aleman-Baez。

摘　　要：The vegetative development of cabbage(Brassica oleracea var.capitata)passes through seedling,rosette,folding and heading stages.Leaves that form the rosette are large and mostly flat.In the following developmental stages,the plants produce leaves that curve inward to produce the leafy head.Many microRNAs and their target genes have been described participating in leaf development and leaf curvature.The aim of this study is to investigate the role of miRNA-regulated genes in the transition from the rosette to the heading stage.We compared the mi RNA and gene abundances between emerging rosette and heading leaves.To remove transcripts(miRNAs and genes)whose regulation was most likely associated with plant age rather than the change from rosette to heading stage,we utilized a non-heading collard green(B.oleracea var.acephala)morphotype as control.This resulted in 33 DEMs and 1998 DEGs with likely roles in the transition from rosette to heading stage in cabbage.Among these 1998 DEGs,we found enriched GO terms related to DNA-binding transcription factor activity,transcription regulator activity,iron ion binding,and photosynthesis.We predicted the target genes of these 33 DEMs and focused on the subset that was differentially expressed(1998DEGs)between rosette and heading stage leaves to construct mi RNA-target gene interaction networks.Our main finding is a role for miR396b-5p targeting two Arabidopsis thaliana orthologues of GROWTH REGULATING FACTORs 3(GRF3)and 4(GRF4)in pointed cabbage head formation.

关 键 词：Brassica oleracea var.capitata RNA sequencing miRNA-target gene network leafy head formation 

分 类 号：S635[农业科学—蔬菜学]