Editing of eIF(iso)4E.c confers resistance against Turnip mosaic virus in Brassica rapa  

作　　者：Yujia Liu Xiaoyun Xin Peirong Li Weihong Wang Yangjun Yu Xiuyun Zhao Deshuang Zhang Jiao Wang Fenglan Zhang Shujiang Zhang Shuancang Yu Tongbing Su 

机构地区：[1]State Key Laboratory of Vegetable Biobreeding,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China [2]National Engineering Research Center for Vegetables,Beijing Key Laboratory of Vegetable Germplasms Improvement,Key Laboratory of Biology and Genetics Improvement of Horticultural Crops(North China),Key Laboratory of Urban Agriculture(North)of Ministry of Agriculture,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China [3]Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Horticultural Plant Journal》2024年第4期1020-1034,共15页园艺学报（英文版）

基　　金：supported by grants from the Scientist Training Program of BAAFS (Grant No.JKZX202406);the Innovation and Capacity-Building Project of BAAFS (Grant No.KJCX20230221);Collaborative innovation program of the Beijing Vegetable Research Center (Grant No.XTCX202302);the National Natural Science Foundation of China (Grant No.32072567);the China Agriculture Research System of MOF and MARA (Grant No.CARS-A03)。

摘　　要：Turnip mosaic virus(TuMV)constitutes one of the primary diseases affecting Brassica rapa,severely impacting its production and resulting in crop failures in various regions worldwide.Recent research has demonstrated the significance of plant translation initiation factors,specifically the eIF4E and eIF4G family genes,as essential recessive disease resistance genes.In our study,we conducted evolutionary and gene expression studies,leading us to identify e IF(iso)4E.c as a potential TuMV-resistant gene.Leveraging CRISPR/Cas9 technology,we obtained mutant B.rapa plants with edited eIF(iso)4E.c gene.We confirmed eIF(iso)4E.c confers resistance against TuMV through phenotypic observations and virus content evaluations.Furthermore,we employed ribosome profiling assays on eif(iso)4e.c mutant seedlings to unravel the translation landscape in response to TuMV.Interestingly,we observed a moderate correlation between the fold changes in gene expression at the transcriptional and translational levels(R^(2)=0.729).Comparative analysis of ribosome profiling and RNA-seq data revealed that plant-pathogen interaction,and MAPK signaling pathway-plant pathways were involved in eIF(iso)4E.c-mediated TuMV resistance.Further analysis revealed that sequence features,coding sequence length,and normalized minimal free energy,influenced the translation efficiency of genes.Our study highlights that the loss of e IF(iso)4E.c can result in a highly intricate translation mechanism,acting synergistically with transcription to confer resistance against TuMV.

关 键 词：Brassica rapa eIF(iso)4E.c TUMV Ribo-seq 

分 类 号：S436.31[农业科学—农业昆虫与害虫防治]