基于UPLC特征图谱和含量测定的不同产地筋骨草质量评价  

Quality Evaluation of Ajuga decumbens from Different Producing Areas Based on UPLC Characteristic Chromatogram and Content Determination

作　　者：丘娴 周湘媛 罗佩琪 彭娇 谢远远 马懿飞 何民友 李振雨 陈向东 孙冬梅 QIU Xian;ZHOU Xiang-yuan;LUO Pei-qi;PENG Jiao;XIE Yuan-yuan;MA Yi-fei;HE Min-you;LI Zhen-yu;CHEN Xiang-dong;SUN Dong-mei(Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China;Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司,广东佛山528244 [2]广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《中国现代中药》2024年第7期1157-1163,共7页Modern Chinese Medicine

基　　金：工业和信息化部2022年产业技术基础公共服务平台项目(2022-230-221);佛山市南海区重点领域科技攻关专项(南科〔2023〕20号-18)。

摘　　要：目的:建立筋骨草超高效液相色谱法(UPLC)特征图谱和其中哈巴苷、乙酰哈巴苷的含量测定方法。方法:采用Waters BEH C18色谱柱(150 mm×2.1 mm,1.7μm),以乙腈-甲醇(4∶1)为流动相A、0.2%磷酸水溶液为流动相B,梯度洗脱,流速为0.30 mL·min^(-1),柱温为30℃,检测波长为202 nm,进样量为2μL,建立筋骨草特征图谱,测定其中哈巴苷、乙酰哈巴苷的含量。结果:17批筋骨草特征图谱有10个共有峰,指认了其中4个成分分别为哈巴苷、乙酰哈巴苷、木犀草素-7-二葡萄糖苷酸、芹菜素-7-二葡萄糖苷酸;17批筋骨草中哈巴苷质量分数为0.33%~0.95%,乙酰哈巴苷质量分数为0.47%~1.93%。结论:建立的UPLC特征图谱和哈巴苷、乙酰哈巴苷含量测定方法可为筋骨草质量评价提供参考。Objective:To establish the characteristic chromatogram of Ajuga decumbens Thunb.by ultra-high performance liquid chromatography(UPLC)and method for determining the content of harpagoside and acetyl harpagoside.Methods:Waters BEH C18(150 mm×2.1 mm,1.7μm)column was used.The mobile phase A was acetonitrile-methanol(4:1),and the mobile phase B was 0.2%phosphoric acid aqueous solution for gradient elution.The flow rate was 0.30 mL·min^(-1),and the column temperature was 30℃.The detection wavelength was 202 nm,and the injection volume was 2μL.The characteristic chromatogram of A.decumbens was established to determine the content of harpagoside and acetyl harpagoside.Results:A total of 10 common fingerprint peaks were identified in the characteristic chromatograms of 17 batches of A.decumbens,among which four components were identified as harpagoside,acetyl harpagoside,luteonin-7-diglucosidic acid,and apigenin-7-diglucosidic acid.The mass fraction of harpagoside in the 17 batches of A.decumbens.was in the range of 0.33%-0.95%,while that of acetyl harpagoside was 0.47%-1.93%.Conclusion:The established UPLC characteristic chromatogram and the method for determining the content of harpagoside and acetyl harpagoside can provide a basis for the quality evaluation of A.decumbens.

关 键 词：筋骨草 超高效液相色谱法 特征图谱 含量测定 

分 类 号：R284[医药卫生—中药学]