广西贺州地区无偿献血人群Mur血型筛查及基因型研究  

Screening and genotyping of Mur blood group among voluntary blood donors in the population of Hezhou,Guangxi

作　　者：袁维权 丁少华[2] 李建敏 吴雪明 文盛名 林厚全 何伟胜 李晓明 张家杰 肖龙明 段生宝[2] 陈盛旺 YUAN Weiquan;DING Shaohua;LI Jianmin;WU Xueming;WEN Shengming;LIN Houquan;HE Weisheng;LI Xiaoming;ZHANG Jiajie;XIAO Longming;DUAN Shengbao;CHEN Shengwang(Hezhou Central Blood Bank,Hezhou 542800,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences;Guangxi Pangeng Health Technology Co.,Ltd;Suzhou Yuanyue Biotechnology Co.,Ltd.)

机构地区：[1]贺州市中心血站,广西贺州542800 [2]中国科学院苏州生物医学工程技术研究所 [3]广西磐庚健康科技有限公司 [4]苏州远跃生物技术有限公司

出　　处：《中国输血杂志》2024年第7期773-778,共6页Chinese Journal of Blood Transfusion

基　　金：“市院科技创新合作专项”项目(22SH16)。

摘　　要：目的 筛查广西贺州地区无偿献血者Mur血型抗原分布频率,并对Mur抗原阳性样本进一步分析其分子基础。方法 采用微孔板血型血清学方法筛查广西贺州地区无偿献血者Mur表型,分析Mur血型抗原在不同少数民族人群中分布频率。对血清学筛查结果阳性样本通过PCR-SSP方法进行基因定型,验证血清学方法的准确性,并测序分析其基因背景。结果 在3 298例贺州地区无偿献血者标本中筛选出Mur抗原阳性432例(13.10%,432/3 298),PCR-SSP基因分型验证显示432例标本均为电泳阳性。其中,汉族献血者Mur抗原阳性占比12.79%(331/2 587),瑶族13.25%(64/483),壮族16.51%(36/218),3组数据无统计学差异(P>0.05)。进一步的测序结果显示,其中428例为GYP(B-A-B)Mur,即GYP.Mur型(12.98%,428/3 298),其余4例为GYP(B-A-B)Bun,即GYP.Bun型(0.12%,4/3 298)。结论 广西贺州地区无偿献血人群Mur血型频率较高,且以GYP.Mur基因型为主。由于民族融合等因素,汉族与壮族、瑶族等少数民族Mur血型分布频率无显著性差异。因此,本地区广泛开展Mur血型抗原抗体检测,对于保障临床输血安全具有重要意义。Objective To screen the distribution frequency of Mur blood group among voluntary blood donors in Hezhou,Guangxi,and further analyze the molecular basis of of Mur antigen positive samples.Methods The Mur phenotype of voluntary blood donors in Hezhou was serologically screened using microplate method,and the distribution frequency of Mur antigens in different ethnic groups was analyzed.Genetic typing was performed on these positive samples with PCR-SSP method to verify the accuracy of the serological method,and the genetic background was sequenced and analyzed.Results Among 3 298 samples from voluntary blood donors in Hezhou,432(13.10%,432/3 298) were screened positive for Mur antigen,and PCR-SSP genotyping validation showed that all 432 samples were electrophoretic positive.Among them,the proportion of Han blood donors with positive Mur antigen was 12.79%(331/2 587),Yao ethnic group was 13.25%(64/483),Zhuang ethnic group was 16.51%(36/218),and no statistically significant difference was found in the three groups(P>0.05).Further sequencing results showed that 428 samples were GYP(B-A-B) Mur,also known as GYP.Mur type(12.98%,428/3 298),the other 4 samples were GYP(B-A-B) Bun,also known as GYP.Bun type(0.12%,4/3 298).Conclusion The Mur blood type frequency is high in the voluntary blood donors in Hezhou,Guangxi,and is predominant characterized by GYP.Mur genotype.Due to ethnic integration,no significant difference was noticed in the frequency of Mur blood type distribution between Han,Zhuang and Yao population.Therefore,conducting extensive Mur blood group antigen and antibody testing in Hezhou is of great significance for ensuring clinical blood transfusion safety.

关 键 词：Mur血型抗原 分布频率 GYP.Mur 输血安全 贺州 

分 类 号：R457.11[医药卫生—治疗学]