改良超低温保存箱制备新鲜冰冻血浆的质量分析  

Quality analysis of fresh frozen plasma prepared by the improved ultra-low temperature storage box

作　　者：周竞 阎兵[1] 戴书明 傅强[1] 达倩倩 艾俊[1] ZHOU Jing;YAN Bing;DAI Shuming;FU Qiang;DA Qianqian;AI Jun(Nanjing Red Cross Blood Center,Nanjing 210003,China)

机构地区：[1]南京红十字血液中心,江苏南京210003

出　　处：《中国输血杂志》2024年第7期816-819,826,共5页Chinese Journal of Blood Transfusion

摘　　要：目的 观察分析使用改良后的超低温保存箱,对制备新鲜冰冻血浆(FFP)的质量影响。方法 选取2023年7—11月采集的400 mL的合格全血标本80例(去除O型),实验分为4组,每组标本20例。A组:使用传统低温冰箱速冻1 h后,放入-30℃冷库储存;B组:使用平板速冻机速冻1 h后,放入-30℃冷库储存;C组:使用改良超低温保存箱速冻1 h后,放入-30℃冷库储存;D组:使用新改良超低温保存箱速冻1 h并储存12 h后,放入-30℃冷库储存。检测4组标本凝血因子FⅧ和纤维蛋白原(Fg)的含量。结果 B、C、D 3组的FⅧ含量均显著高于A组,有统计学差异(P<0.05),B、C、D 3组之间FⅧ含量差异无统计学意义(P>0.05),4组之间的Fg含量差异均没有统计学意义(P>0.05)。结论 新改良的超低温保存箱在制备FFP的质量上优于低温冰箱的传统制备工艺,与平板速冻机制备效果差异无统计学意义,但改良式的超低温保存箱兼顾储存功能,可以使FFP的制备流程更加灵活,可以提高成分制备工作的效率。Objective To observe and analyze the influence of the improved ultra-low temperature storage box on the quality of fresh frozen plasma(FFP).Methods A total of 80 qualified whole blood samples(400 mL,O type not included) collected from July to November in 2023 were selected,and were divided into 4 groups,with 20 samples in each group.Group A:quick-frozen in a traditional low temperature box for 1 hour and then stored in a-30℃ cold storage;Group B:quick-frozen in the flat freezer for 1 hour and then stored in a-30℃ cold storage;Group C:quick-frozen in a newly improved ultra-low temperature storage box for 1 hour and stored in a-30℃ cold storage;Group D:quick-frozen in a new improved ultra-low temperature storage box for 12 hours and stored in a-30℃ cold storage.The contents of FⅧ and fibrinogen(Fg) in four groups were detected.Results The contents of FⅧ in group B,C and D were significantly higher than those in group A,with statistical difference(P<0.05),but with no statistical difference among gourp B,C and D(P>0.05),and no statistical difference in the contents of Fg was found among the four groups(P>0.05).Conclusion The improved ultra-low temperature storage box is superior to the traditional low temperature box in preparing FFP,and there is no obvious difference between the improved ultra-low temperature storage box and the flat-plate quick freezer.However,the improved ultra-low temperature storage box can make the process of preparing FFP more flexible and improve the efficiency of component preparation.

关 键 词：新鲜冰冻血浆 FⅧ 超低温保存箱 

分 类 号：R457.14[医药卫生—治疗学]