4℃保存全血制备的去白细胞混合浓缩血小板体外质量评价  

In vitro quality evaluation of leucocyte-reduced pooled platelet concentrates prepared from whole blood stored at 4℃

作　　者：安慧娟[1] 单泓[1] 刘铮[1] 张姣姣 王姣杰[1] 别立莉[1] 李建斌[1] 金新莉[1] AN Huijuan;SHAN Hong;LIU Zheng;ZHANG Jiaojiao;WANG Jiaojie;BIE Lili;LI Jianbin;JIN Xinli(Henan Red Cross Blood Center,Zhengzhou 450012,China)

机构地区：[1]河南省红十字血液中心,河南郑州450012

出　　处：《中国输血杂志》2024年第7期820-826,共7页Chinese Journal of Blood Transfusion

基　　金：中国输血协会威高科研基金资助项目(CSBT-WG-2021-01);河南省医学科技攻关计划联合共建项目(LHGJ20200252)。

摘　　要：目的 探讨全血4℃冷藏条件下保存制备去白细胞混合浓缩血小板的可行性,为成分制备提供理论依据。方法 将采集的400 mL ACD-B抗凝全血随机分两组,分别进行4℃和室温保存,在保存后6 h内分离制备出白膜层,于22℃静置过夜保存,次日将白膜层汇集制备去白细胞混合浓缩血小板,于制备后d1、d3、d5、d7取样,进行血细胞计数及相关参数检测,测定pH、葡萄糖、乳酸含量反映代谢情况,检测血栓弹力图、血小板聚集率及PAC-1、CD62P反映血小板体外功能及活化情况,比较两组血小板差异。结果 随着保存时间的延长,两组去白细胞混合浓缩血小板计数逐渐下降,血小板分布宽度(PDW)、平均血小板体积(MPV)、大型血小板比例(P-LCR)均逐渐上升,差异无统计学意义(P>0.05)。两组去白细胞混合浓缩血小板pH、葡萄糖含量均逐渐降低,乳酸含量逐渐增高,无显著性差异(P>0.05)。血栓弹力图结果显示,反应血小板功能的MA值保存期内无显著性变化,且两组间无显著性差异(P>0.05)。血小板聚集率随着保存时间的延长而逐渐降低,两组无统计学差异。血小板PAC-1及CD62P表达率随着保存时间的延长而逐渐升高,两组无统计学差异(P>0.05)。结论 全血4℃冷藏保存与室温保存6 h内制备的去白细胞混合浓缩血小板在体外计数、功能、活化方面无统计学差异,4℃冷藏6 h内的全血可考虑作为制备去白细胞混合浓缩血小板的起始血液。Objective To investigate the feasibility of leucocyte-reduced pooled platelet concentrates from whole blood stored at 4℃,and provide theoretical basis for the components preparation.Methods The collected 400 mL ACD-B anticoagulant whole blood was randomly divided into two groups,stored at 4℃ and room temperature.The buffy coat was prepared within 6 hours and store at 22℃ until next day to prepare leucocyte-reduced pooled platelet concentrates.Platelet samples on day 1,3,5 and 7 were taken for the blood cell count and related parameter detection.The pH,glucose and lactic acid content were determined to reflect the metabolic status,and the thromboelastography,platelet aggregation rate and PAC-1 and CD62P expression were determined to reflect the function and activation of platelets.The difference in platelets between two groups were analyzed.Results With the extension of storage time,the count of leucocyte-reduced pooled platelet concentrates decreased gradually,but the platelets distribution width(PDW),mean platelet volume(MPV) and platelet-larger cell ratio(P-LCR) increased gradually in two groups,with no statistical significance(P>0.05).The pH and glucose contents in two groups gradually decreased,but the lactic acid content gradually increased,with no significant difference(P>0.05).The thrombelastogram showed MA value that reflecting platelet function has no significant change during the storage,and there was no significant difference between the two groups(P>0.05).The aggregation rates decreased while the expression of PAC-1 and CD62P increased gradually with the prolongation of preservation time,with no significant difference between the two groups(P>0.05).Conclusion There is no significant difference in platelet count,function and activation between whole blood stored at 4℃ and at room temperature within 6 hours.Whole blood stored at 4℃ within 6 hours can be considered as the raw material for leucocyte-reduced pooled platelet concentrates.

关 键 词：去白细胞混合浓缩血小板 全血冷藏保存 血小板质量 血小板功能 

分 类 号：R457.14[医药卫生—治疗学] R331.143[医药卫生—临床医学]