紫草银纳米粒的绿色合成及抗浅部真菌作用研究  

Green Synthesis of Silver Nanoparticles of Arnebia Euchroma and Their Anti-superficial Fungi Effects

作　　者：杨雯雯 叶明琦 张敏新 黄惠丽[1] 邱彬 黄爱文 YANG Wen-wen;YE Ming-qi;ZHANG Min-xin;HUANG Hui-li;QIU Bin;HUANG Ai-wen(Clinical Pharmacy Department,Fujian University of Traditional Chinese Medicine Fuzong Teaching Hospital(900th hospital),Fuzhou,Fujian,350025,China;College of Pharmacy,Fujian University of Traditional ChineseMedicine,Fuzhou,Fujian,350122,China;College of Chemistry,Fuzhou University,Fuzhou,Fujian,350108,China)

机构地区：[1]福建中医药大学福总教学医院(第九〇〇医院)临床药学科,福建福州350025 [2]福建中医药大学药学院,福建福州350122 [3]福州大学化学学院,建福州350108

出　　处：《现代生物医学进展》2024年第12期2228-2236,共9页Progress in Modern Biomedicine

基　　金：福建省自然科学基金项目(2021J011274)。

摘　　要：目的:以紫草提取液为还原剂,以硝酸银为银源,简单快速地合成银纳米粒子,并对其进行表征和抗浅部真菌作用研究。方法:以合成银纳米粒的吸光度为指标,考察紫草提取液用量、AgNO_(3)溶液浓度、反应pH对紫草银纳米粒(ZC-AgNPs)合成的影响,应用响应面优化筛选最佳合成条件。采用紫外-可见分光光度法(UV-Vis)、动态激光散射(DLS)、透射电子显微镜(TEM)、X射线粉末衍射(XRD)、傅里叶变换红外光谱(FTIR)等技术对紫草-银纳米粒的理化性质进行表征。同时利用化学还原法制备化学银纳米粒(C-AgNPs),利用微量稀释法测试ZC-AgNPs和C-AgNPs对浅部真菌的最低抑菌浓度(MIC)。结果:ZC-AgNPs的最佳工艺为硝酸银浓度10 mmol/L、紫草提取液体积1.7 mL、反应pH为10.8。所得ZC-AgNPs形状为球形,透射电子显微镜分析平均粒径约为12.59 nm,分布均匀、性质稳定。抗浅部真菌实验结果显示ZC-AgNPs均优于C-AgNPs,ZC-AgNPs对红色毛癣菌MIC为62.5μg/mL、对须癣毛癣菌MIC为125μg/mL、对白色念珠菌的MIC为15.63μg/mL;C-AgNPs对红色毛癣菌MIC为250μg/mL,对须癣毛癣菌MIC为500μg/mL,对白色念珠菌的MIC为250μg/mL。结论:ZC-AgNPs制备方法稳定、可行,且具有良好的抗浅部真菌效果。Objective:Simple and rapid synthesis of silver nanoparticles using Arnebia euchroma extract as a reducing agent and silver nitrate as a silver source for characterization and anti-superficial fungi effects.Methods:The absorbance of the synthesized silver nanoparticles was used as an index to investigate the effects of the amount of comfrey extract,the concentration of AgNO_(3) solution,and reaction pH on the synthesis of silver nanoparticles of A.euchroma(ZC-AgNPs),and the response surface optimization was applied to screen the optimal synthesis conditions.The physicochemical properties of ZC-AgNPs were characterized by ultraviolet-visible spectrophotometry(UV-Vis),dynamic laser scattering(DLS),transmission electron microscopy(TEM),X-ray powder diffraction(XRD),and Fourier-transform infrared spectroscopy(FTIR).Chemical silver nanoparticles(C-AgNPs)were also prepared by the chemical reduction method,and the minimum inhibitory concentration(MIC)of ZC-AgNPs and C-AgNPs against superficial fungi was tested using the microdilution method.Results:The optimal process for ZC-AgNPs was a silver nitrate concentration of 10 mmol/L,a volume of comfrey extract 1.7 mL,and a pH of 10.8.The obtained ZC-AgNPs were spherical,with an average particle size of about 12.59 nm analyzed by transmission electron microscopy,and were uniformly distributed and stable.The results of the antifungal assay against superficial fungi showed that all ZC-AgNPs were superior to C-AgNPs.The MIC of ZC-AgNPs against Trichophyton rubrum,T.mentagrophyte,and Candida albicans were 62.5,125,and 15.63μg/mL,respectively.The MIC of C-AgNPs against T,rubrum,T.mentagrophytes,and Candida albicans were 250,500,and 250μg/mL,respectively.Conclusions:The preparation method of ZC-AgNPs is stable,feasible,and has a good antifungal effects against superficial fungi.

关 键 词：绿色合成 银纳米粒 紫草 浅部真菌 

分 类 号：R283[医药卫生—中药学] R519[医药卫生—中医学] R-33