中药金莲花的体外遗传毒性评价  

Preliminary Genotoxic Study of Trollius Chinensis Bunge

作　　者：唐慧[1] 程东[1] TANG Hui;CHENG Dong(Toxicology Laboratory,Department of Health Inspection and Testing,Shandong Center for Disease Control and Prevention,Jinan,Shandong250014,China)

机构地区：[1]山东省疾病预防控制中心卫生检验检测所毒理室,山东济南250014

出　　处：《预防医学论坛》2024年第4期268-271,共4页Preventive Medicine Tribune

摘　　要：目的 对中药金莲花的体外遗传毒性进行评价,为其在临床治疗和药物研制开发提供毒理学依据。方法 选择冻干鼠伤寒沙门氏菌组氨酸缺陷型菌株TA97a、TA98、TA100、TA102、TA1535,各菌株均设置试验组、验证组、未处理对照组、纯化水对照组、二甲基亚砜对照组和阳性对照组。采用细菌回复突变试验,试验组每皿加入0.1 mL金莲花溶液,其质量浓度分别相当于5 000、1 563、488、153、48 μg/皿。阳性对照组所用阳性物根据菌株敏感性不同,分别选用敌克松(Dexon, 50 μg/皿)、2-氨基芴(2-AF,10 μg/皿)、甲基磺酸甲酯(MMC,0.5 μL/皿)、1,8-二羟基蒽醌(Dionone, 50 μg/皿)、叠氮化钠(NaN_3,0.25 μg/皿)、2-氨基蒽(2-AA,1 μg/皿),各阳性物均以二甲基亚砜为溶剂配制。未处理对照组只在顶层培养基中加入同体积菌液,纯化水对照组加入同体积无菌纯化水。验证组金莲花剂量为8、40、200、1 000、5 000 μg/皿5个剂量。采用平板掺入法,以大鼠肝微粒体酶S9作为代谢活化系统,计数金莲花对5株鼠伤寒沙门菌组氨酸缺陷型菌株作用后的回复突变菌落数。结果 试验组、纯化水对照组和二甲基亚砜对照组各测试菌株的回变菌落数与未处理对照组菌株的自发回变菌落数相比均未有≥1倍数量的增加,未呈现剂量-反应关系,亦无某一测试点有可重复性的阳性结果;阳性对照组各标准诱变剂诱发的回复突变菌落数均超过未处理对照组菌落数的2倍以上。在有/无代谢活化系统的条件下,金莲花对未诱发测试菌株TA97a、TA98、TA100、TA102、TA1535基因突变,未见有遗传毒性表现。结论 在本试验条件下,未见金莲花有体外致基因突变的作用。Objective To evaluate the genetic toxicity of Trollius Chinensis Bunge,so as to provide a toxicological basis for its use in clinical treatment and drug development.Methods Lyophilised Salmonella Typhimurium histidine-deficient strains TA97a,TA98,TA100,TA102and TA1535were selected,and experimental group,verification group,untreated control group,purified water control group,DMSO control group and positive control group were set up for each strain.A bacterial revert mutation test was used,where 0.1mL of Trollius Chinensis Bunge solution was added to each dish of the test group at a mass concentration equivalent to 5000,1563,488,153,and 48μg/dish,respectively.The standard mutagen used in the positive control group were selected according to the sensitivity of the strains,and were Dexon(50μg/dish),2-Aminofluorene(2-AF,10μg/dish),Methyl methanesulfonate(MMC,0.5μL/dish),1,8-Dihydroxyanthraquinone(Dionone,50μg/dish),Sodium azide(NaN3,0.25μg/dish),and 2-Aminoanthracene(2-AA,1μg/dish),each of which was prepared with dimethylsulfoxide as a solvent.Amino anthracene(2-AA,1μg/dish),and each positive was formulated with dimethyl sulfoxide as solvent.In the verification test,the dosages were 8,40,200,1000and 5000μg/dish.Using the liver microsomal enzyme S9as a metabolic activation system,the number of revertant colonies of 5strains of Salmonella typhimurium were counted.Results Compared with the untreated control group,the tested group,purified water control group and DMSO control group,the number of revertant colonies did not increase by more than 1times,and there was no dose-response relationship,and no repeatable positive result at any test point.The number of revertant colonies induced by each standard mutagen in the positive control group was more than twice that in the untreated control group.With or without metabolic activation system,the strain TA97a,TA98,TA100,TA102,TA1535did not induce gene mutation,and no genotoxicity was observed.Conclusion Under the experimental conditions,Trollius Chinensis Bunge has no

关 键 词：金莲花 细菌回复突变试验 遗传毒性 

分 类 号：R285.5[医药卫生—中药学] R394.6[医药卫生—中医学]