猴痘病毒分子生物学检测方法的研究进展  

Advances in study of molecular identification technology of monkeypox virus

作　　者：王雯雯 WANG Wen-wen(Laboratory,Liangzhou District Center for Disease Control and Prevention,Wuwei,Gansu733000,China)

机构地区：[1]凉州区疾病预防控制中心实验室,甘肃武威733000

出　　处：《预防医学论坛》2024年第4期316-320,共5页Preventive Medicine Tribune

摘　　要：猴痘是一种由猴痘病毒(MPXV)感染所致的人兽共患病毒性疾病。2022年以来,世界多国出现猴痘疫情,并呈快速扩散态势。因此,在感染初期检测出MPXV并对患者及时采取有效的隔离措施、治疗,对于控制疫情传播尤为重要。近年来,国内外学者建立了多种MPXV分子检测方法,主要包括聚合酶链式反应(PCR)、荧光定量PCR(Real-time PCR)、多重荧光定量PCR(mRT-PCR)、数字PCR(dPCR)和核酸等温扩增等。本文现就MPXV分子诊断检测方法的技术原理、特点、应用场景以及优缺点等综述,以期为开发更高效、更准确的MPXV检测方法提供新思路,并对MPXV未来研究方向和防控策略提供参考和建议。Monkeypox is a zoonotic disease caused by monkeypox virus(MPXV)infection.Since 2022,multiple countries around the world have experienced monkeypox outbreaks,which have shown a rapid spread trend.Therefore,it is particularly important to detect MPXV at the early stage of infection to take effective isolation measures and treatment in time to control the spread of the epidemic.Domestic and foreign scholars have established a variety of molecular detection techniques for the MPXV over recent years,mainly including polymerase chain reaction(PCR),real-time PCR(RT-PCR),multiple reai-time PCR(mRT-PCR),digital PCR(dPCR),and nucleic acid isothermal amplification technology.In this article,the technical principle,characteristics,application scenarios,advantages and disadvantages of MPXV molecular diagnostic detection method were reviewed,with a view to providing new ideas for the detection of MPXV efficiently and accurately,and also providing references and suggestions for future research directions and prevention and control strategies of MPXV.

关 键 词：猴痘 病毒 分子检测技术 核酸 PCR 诊断 

分 类 号：R373[医药卫生—病原生物学] R446[医药卫生—基础医学]