The RNA binding protein EHD6 recruits the m^(6)A reader YTH07 and sequesters OsCOL4 mRNA into phase-separated ribonucleoprotein condensates to promote rice flowering  

作　　者：Song Cui Peizhe Song Chaolong Wang Saihua Chen Benyuan Hao Zhuang Xu Liang Cai Xu Chen Shanshan Zhu Xiangchao Gan Hui Dong Yuan Hu Liang Zhou Haigang Hou Yunlu Tian Xi Liu Liangming Chen Shijia Liu Ling Jiang Haiyang Wang Guifang Jia Shirong Zhou Jianmin Wan 

机构地区：[1]State Key Laboratory for Crop Genetics&Germplasm Enhancement and Utilization,Zhongshan Biological Breeding Laboratory,National Observation and Research Station of Rice Germplasm Resources,Nanjing Agricultural University,Nanjing 210095,China [2]State Key Laboratory for Crop Gene Resources and Breeding,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,College of Chemistry and Molecular Engineering,Peking-Tsinghua Center for Life Sciences,Beijing Advanced Center of RNA Biology,Peking University,Beijing,China [4]Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Agricultural College of Yangzhou University,Yangzhou 225009,China [5]Max Planck Institute for Plant Breeding Research,Carl-von-Linne´-Weg 10,50829 Koln,Germany

出　　处：《Molecular Plant》2024年第6期935-954,共20页分子植物（英文版）

基　　金：supported by the Key Laboratory of Biology,Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River,Ministry of Agriculture and Rural Affairs,China,and the Jiangsu Collaborative Innovation Center for Modern Crop Production,China.Funding for this work was provided by the National Key Research and Development Program of China(2020YFE0202300 and 2021YFD1200504);the National Natural Science Foundation of China(31971910 and 32272115);the National Science Foundation of Jiangsu Province(BK20212010 and BK20230038);the Foundation of Biological Breeding Zhongshan Lab(BM2022008-03,ZSBBL-KY2023-04,and ZSBBL-KY2023-06).

摘　　要：N6-Methyladenosine(m^(6)A)is one of the most abundant modifications of eukaryotic mRNA,but its comprehensive biological functionality remains further exploration.In this study,we identified and characterized a new flowering-promoting gene,EARLY HEADING DATE6(EHD6),in rice.EHD6 encodes an RNA recognition motif(RRM)-containing RNA binding protein that is localized in the non-membranous cytoplasm ribonucleoprotein(RNP)granules and can bind both m^(6)A-modified RNA and unmodified RNA indiscriminately.We found that EHD6 can physically interact with YTH07,a YTH(YT521-B homology)domain-containing m^(6)A reader.We showed that their interaction enhances the binding of an m^(6)A-modified RNA and triggers relocation of a portion of YTH07 from the cytoplasm into RNP granules through phase-separated condensation.Within these condensates,the mRNA of a rice flowering repressor,CONSTANS-like 4(OsCOL4),becomes sequestered,leading to a reduction in its protein abundance and thus accelerated flowering through the Early heading date 1 pathway.Taken together,these results not only shed new light on the molecular mechanism of efficient m^(6)A recognition by the collaboration between an RNA binding protein and YTH family m^(6)A reader,but also uncover the potential for m^(6)A-mediated translation regulation through phaseseparated ribonucleoprotein condensation in rice.

关 键 词：N6-methyladenosine m^(6)A EHD6 YTH domain-containing protein phase separation heading date 

分 类 号：Q94[生物学—植物学]