四川省4个主栽核桃品种对炭疽病的生理生化响应  

Physiological and biochemical responses to anthracnose diseaseof four main walnut cultivars in Sichuan Province

作　　者：李润 董茂坤 吴雨桦 刘华[1] 李丕军[2] 邢文曦 冯春[1] LI Run;DONG Maokun;WU Yuhua;LIU Hua;LI Pijun;XING Wenxi;FENG Chun(School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036;Sichuan Academy of Forestry,Chengdu 610081)

机构地区：[1]安徽农业大学林学与园林学院,合肥230036 [2]四川省林业科学研究院,成都610081

出　　处：《安徽农业大学学报》2024年第3期411-418,共8页Journal of Anhui Agricultural University

基　　金：四川省科技厅重点研发项目“突破性林木育种材料和方法创新及新品种选育”子专题(2021YFYZ0032);四川省基本科研项目(2021JBKY07)共同资助。

摘　　要：以四川省栽培面积较大的4个品种,‘旺核2号’、‘川核66’、‘盐源早’和‘硕星’为研究对象,进行人工侵染炭疽病,测定染病后叶片发病特征与相关生理生化指标,最后综合评价其对炭疽病的抗性。结果表明:随炭疽病侵染时间延长,4个品种的叶片发病率、病斑面积、新增病斑扩增比例以及病情指数均呈现先增后减的趋势,其中发病率、病斑面积最大的是‘旺核2号’和‘硕星’,最小的是‘盐源早’;叶绿素含量与CAT活性均呈现不断下降的趋势,MDA含量、POD活性、SOD活性呈上升或下降的趋势。相关性分析以及灰色关联度结果表明,4个品种的POD含量与病斑面积、新增病斑面积扩增比例和病情指数呈极显著正相关(P<0.01),叶绿素含量与病斑面积、发病率、新增病斑面积扩增比例及病情指数呈极显著负相关(P<0.01);核桃品种各项抗病性指标的综合关联度顺序为POD活性>MDA含量>SOD活性>CAT活性>叶绿素含量。In this study,taken four varieties with relatively large cultivation areas in Sichuan Province,i.e.,‘Wanghe 2’,‘Chuanhe 66’,‘Yanyuanzao’,and‘Shuoxing’as the research objects,an artificial infection with anthracnose was carried out,the leaf disease characteristics and related physiological and biochemical indicators were measured after infection,and the resistance to anthracnose was comprehensively evaluated.The results showed that:as the infection time of anthrax prolonged,the leaf incidence rate,disease spot area,expansion ratio of new disease spots and disease index of the four varieties showed a trend of increasing first and then decreasing,among them,the largest incidence and disease spot area were‘Wanghe 2’and‘Shuoxing’,and the smallest was‘Yanyuanzao’;the chlorophyll content and CAT activity showed a continuous decreasing trend,while the MDA content,POD activity and SOD activity showed an increasing or decreasing trend.The results of correlation analysis and grey correlation degree showed that the POD activities in the four varieties were significantly positively correlated with the disease spot area,the expansion ratio of new disease spot area and disease index(P<0.01),while their chlorophyll contents were significantly negatively correlated with the disease spot area,the incidence rate,the expansion ratio of new disease spot area and disease index(P<0.01);the comprehensive correlation order of various disease resistance indicators in the four walnut varieties was POD activity>MDA content>SOD activity>CAT activity>chlorophyll content.

关 键 词：核桃炭疽病 抗性评价 生理生化 病情指数 

分 类 号：S664.1[农业科学—果树学] S436.64[农业科学—园艺学]