零价铁对猪粪发酵产甲烷和磷转化的影响  

Effect of zero-valent iron on methane production and phosphorusconversion during pig manure digestion

作　　者：胡良慧 鲍静 李海铭 张田田 夏明强 梁越敢[1] HU Lianghui;BAO Jing;LI Haiming;ZHANG Tiantian;XIA Mingqiang;LIANG Yuegan(School of Resources and Environment,Anhui Agricultural University,Hefei 230036;Anhui Wanxin Environmental Technology Co.,Ltd.,Hefei 230093)

机构地区：[1]安徽农业大学资源与环境学院,合肥230036 [2]安徽皖欣环境科技有限公司,合肥230093

出　　处：《安徽农业大学学报》2024年第3期481-487,共7页Journal of Anhui Agricultural University

基　　金：十三五巢湖水专项(2017ZX07603-004)资助。

摘　　要：为解决猪粪发酵沼液中有机物和磷对环境造成的污染问题,提高清洁能源的生产率,通过在厌氧发酵系统中投加零价铁,采用傅里叶红外光谱仪(Fourier transform infrared spectrometer,FTIR)、X射线衍射分析(X-ray diffraction,XRD)、高通量测序等技术分析厌氧发酵过程中产甲烷量、磷的分布与形态、酶活性以及微生物群落等变化,研究零价铁对猪粪厌氧发酵产甲烷和磷转化的影响。结果表明,零价铁富集了Clostridun_sensu_stricto_1、Terripotobacter和Syntrophomonas等酸化菌,促进了水解酶的活性,使蛋白酶和β-葡萄糖苷酶的活性分别提高了0.6倍和2.4倍,增强了系统的水解酸化能力。并且零价铁还富集了产甲烷菌Methanosaeta,使产甲烷菌活性增强,最终提高了产甲烷11.1%。另外,零价铁富集了Ruminofilibacter和WS6_Dojkabacteria等相关磷转化的微生物,使酸(碱)性磷酸酶的活性增强26.3%左右,促进了系统内有机磷向无机磷转化。同时,零价铁显著提高了系统中溶解性Fe2+的含量,促进了水溶性磷向Fe结合磷化物的转化,最终固定在蓝铁矿等沉淀物中,显著地降低沼液总磷浓度。研究结果为提高猪粪厌氧发酵产甲烷和磷的固定提供了技术参考。In order to solve the environmental pollution caused by organic matter and phosphorus in the biogas slurry of pig manure and to improve the generation of clean energy,the effects of zero-valent iron on methane production and phosphorus transformation were investigated during the anaerobic digestion of pig manure in this study.Techniques such as Fourier transform infrared spectrometer(FTIR),X-ray diffraction(XRD),and high-throughput sequencing were used to analyze the changes in methane production,phosphorus distribution and species,enzyme activities,and microbial community during anaerobic digestion.The results showed that zero-valent iron enriched acidifying bacteria such as Clostridun_sensu_stricto_1,Terripotobacter and Syntrophomonas,and the activities of protease andβ-glucosidase were increased by 0.6 times and 2.4 times,respectively after adding zero-valent iron,and strengthened the hydrolytic acidification ability of the system.Moreover,zero-valent iron enriched Methanosaeta,which was help to enhance the activity of methanogen,and thus the methane production increased by 11.1%.In addition,zero-valent iron also enriched phosphorus transforming microorganisms,such as Ruminofilibacter,WS6_Dojkabacteria etc.,which increased the activities of acid(alkali)phosphatase by about 26.3%,and promoted the conversion of organic phosphorus to inorganic phosphorus in the system.Moreover,zero-valent iron significantly increased the content of soluble Fe2+in the system,and improved the conversion of water-soluble phosphorus to Fe-bound phosphorus,which was finally fixed in the precipitate such as vivianite,and thus significantly reduced the concentration of total phosphorus in biogas slurry.The results provide a technical reference for improving methane production and phosphorus fixation in anaerobic digestion of pig manure.

关 键 词：猪粪 厌氧发酵 零价铁 磷 产甲烷 

分 类 号：X713[环境科学与工程—环境工程]