Plant triterpenoid saponins function as susceptibility factors to promote the pathogenicity of Botrytis cinerea  

作　　者：Francisco J.Escaray Amelia Felipo-Benavent Cristian J.Antonelli Begona Balaguer Maria Pilar Lopez-Gresa Pablo Vera 

机构地区：[1]Instituto de Biologia Molecular y Celular de Plantas,Universidad Politecnica de Valencia-C.S.l.C,Ciudad Politecnica de la Innovacion,Edificio 8E,acceso G,Ingeniero Fausto Elio,s/n,46022Valencia,Spain

出　　处：《Molecular Plant》2024年第7期1073-1089,共17页分子植物（英文版）

基　　金：grants TED2021-130979B-100 and PID2021-1261510B-I00 to P.V.from the Spanish AEl research agency。

摘　　要：The gray mold fungus Botrytis cinerea is a necrotrophic pathogen that causes diseases in hundreds of plant species,including high-value crops.Its polyxenous nature and pathogenic success are due to its ability to perceive host signals in its favor.In this study,we found that laticifer cells of Euphorbia lathyris are a source of susceptibility factors required by B.cinerea to cause disease.Consequently,poor-in-latex(pil)mutants,which lack laticifer cells,show full resistance to this pathogen,whereas lot-of-latex mutants,which produce more laticifer cells,are hypersusceptible.These S factors are triterpenoid saponins,which are widely distributed natural products of vast structural diversity.The downregulation of laticifer-specific oxydosqualene cyclase genes,which encode the first committed step enzymes for triterpene and,therefore,saponin biosynthesis,conferred disease resistance to B.cinerea.Likewise,the Medicago truncatula Iha-1 mutant,compromised in triterpenoid saponin biosynthesis,showed enhanced resistance.Interestingly,the application of different purified triterpenoid saponins pharmacologically complemented the diseaseresistant phenotype ofpil and hla-1 mutants and enhanced disease susceptibility in different plant species.We found that triterpenoid saponins function as plant cues that signal transcriptional reprogramming in B.cinerea,leading to a change in its growth habit and infection strategy,culminating in the abundant formation of infection cushions,the multicellular appressoria apparatus dedicated to plant penetration and biomass destruction in B.cinerea.Taken together,these results provide an explanation for how plant triterpenoid saponins function as disease susceptibility factors to promote B.cinerea pathogenicity.

关 键 词：Euphorbia lathyris laticifer cell LATEX APPRESSORIUM infection cushion necrotrophic fungi 

分 类 号：Q94[生物学—植物学]