Two MADS-box proteins,AGL9 and AGL15,recruit the FIS-PRC2 complex to trigger the phase transition from endosperm proliferation to embryo development in Arabidopsis  

作　　者：Shen Zhang Devasantosh Mohanty Adnan Muzaffar Min Ni 

机构地区：[1]Department of Plant and Microbial Biology,University of Minnesota at Twin Cities,Saint Paul,Minnesota MN 55108,United States of AmericaUSA

出　　处：《Molecular Plant》2024年第7期1110-1128,共19页分子植物（英文版）

基　　金：a grant from National Science Foundation IOS-1933291(M.N.).

摘　　要：Spatiotemporal regulation of gene expression by polycomb repressive complex 2(PRc2)is critical for animal and plant development.The Arabidopsis fertilization independent seed(FIS)-PRC2 complex functions specifically during plant reproduction from gametogenesis to seed development.After a double fertilization event,triploid endosperm proliferates early,followed by the growth of a diploid embryo,which replaces the endosperm in Arabidopsis and many dicots.Key genes critical for endosperm proliferation such as IKkU2 and MINI3 are activated after fertilization.Here we report that two MADS-box AGAMOUS-LIKE(AGL)proteins associate with the key endosperm proliferation loci and recruit the FIS-PRC2 repressive complex at 4-5 days after pollination(DAP).Interestingly,AGL9 and AGL15 only accumulate toward the end of endosperm proliferation at 4-5 DAP and promote the deposition of H3K27me3 marks at key endosperm proliferation loci.Disruption of AGL9 and AGL15 or overexpression of AGL9 or AGL15 significantly influence endosperm proliferation and cellularization.Genome-wide analysis with cleavage Under Targets and tagmentation(CUT&Tag)sequencing and RNA sequencing revealed the landscape of endosperm H3K27me3 marks and gene expression profiles in Col-0 and agl9 agl15.CUT&Tag qPCR also demonstrated the occupancy of the two MADS-box proteins and FIS-PRC2 on a few representative target loci.Our studies suggest that MADS-box proteins could potentially recruit PRc2 to regulate many other developmental processes in plants or even in fungi and animals.

关 键 词：AGL9 AGL15 EMBRYO ENDOSPERM IKU2 MEDEA MINI3 

分 类 号：Q94[生物学—植物学]