黄花倒水莲花的化学成分及体外降胆固醇活性研究  

Chemical Constituents from Flowers of Polygala fallax and Their Cholesterol-Lowering Activity in Vitro

作　　者：薛佳丽 李连春 潘争红[2] 谭兰芳 宁德生[2] XUE Jiali;LI Lianchun;PAN Zhenghong;TAN Lanfang;NING Desheng(College of Pharmacy,Guilin Medical University,Gulin 541004,China;Guangxi Key Laboratory of Plant Functional Substances and Sustainable Utilization of Resources,Guangxi Institute of Botany,Chinese Academy of Sciences,Guangxi Zhuang Autonomous Region,Guilin 541006,China)

机构地区：[1]桂林医学院药学院,广西桂林541004 [2]广西壮族自治区、中国科学院广西植物研究所广西植物功能物质与资源持续利用重点实验室,广西桂林541006

出　　处：《生物化工》2024年第3期40-44,共5页Biological Chemical Engineering

基　　金：国家自然科学基金(82360685);广西科技重大专项(桂科AA22096020);广西林业科技推广示范项目(桂林科研[2022]第3号);中国科学院西部青年学者(人字〔2023〕16号);桂林市创新平台和人才计划(20210102-3)。

摘　　要：为了研究黄花倒水莲花的化学成分及其降胆固醇活性,本文采用硅胶、MCI、Sephadex LH-20和高效液相色谱等技术对黄花倒水莲花的95%乙醇提取物低极性部位进行分离纯化,共得到11个化合物,根据波谱数据及文献比对,分别鉴定为3,4-二甲氧基肉桂酸(1)、1,3,6-三羟基-2,7-二甲氧基口山酮(2)、7-羟基-1-甲氧基-2,3-亚甲二氧基口山酮(3)、1,7-二羟基-2,3-亚甲二氧基口山酮(4)、1-甲氧基-2,3-亚甲二氧基口山酮(5)、1,3-二羟基-2-甲氧基口山酮(6)、(2S,3S,4R,10E)-2-[(2R)-2-羟基-二十四烷酰氨基]-10-十八烷-1,3,4-三醇(7)、楤木脑苷脂(8)、波甾醇-3-O-β-D-吡喃葡萄糖苷(9)、棕榈酸(10)、硬脂酸(11),其中化合物7和9为首次从该植物中分离得到。采用试剂盒法测定所获得化合物对HepG2细胞胆固醇含量的影响,活性筛选结果显示化合物8具有良好的降胆固醇活性,半抑制浓度(IC_(50))为40.3µmol/L,可作为降胆固醇药物研发的候选分子。This work is to investigate the chemical constituents and their cholesterol-lowering activity of flowers of Polygala fallax.Eleven compounds are isolated and purified through silica gel,MCI,Sephadex LH-20 and highperformance liquid chromatography(HPLC)from the low polarity components of 95%ethanol extract of flowers of Polygala fallax.Based on the spectral data and literature comparison,their chemical structures are identified as 3,4-dimethoxy cinnamic acid(1),1,3,6-trihydroxy-2,7-dimethoxyxanthone(2),7-hydroxy-1-methoxy-2,3-methylenedioxyxanthone(3),1,7-dihydroxy-2,3-methylenedioxyxanthone(4),1-methoxy-2,3-methylenedioxyxanthone(5),1,3-dihydroxy-2-methoxyxanthone(6),(2S,3S,4R,10E)-2-[(2R)-2-hydroxyl-tetracosanoly-amino]-10-octadecene-1,3,4-triol(7),aralia cerebroside(8),3-O-β-D-glucopyranosyl spinasterol(9),palmitic acid(10),stearic acid(11),respectively.Compounds 7 and 9 are isolated from this plant for the first time.The effect of the obtained compounds on the cholesterol content are determined using the kit method in HepG2 cells,and the activity screening results show that compound 8 has good cholesterol-lowering activity with an half maximal inhibitory concentration(IC_(50))of 40.3μmol/L,which can be used as a candidate molecule for the development of cholesterol-lowering drugs.

关 键 词：黄花倒水莲 花 化学成分 降胆固醇活性 

分 类 号：R932[医药卫生—生药学] O657[医药卫生—药学]