Naked-eye visualization of lymph nodes using fluorescence nanoprobes in non-human primate-animal models  

作　　者：Xiaoyuan Ji Binbin Chu Xiaofeng Wu Zhiming Xia Airui Jiang Chenyu Wang Zhiming Chen Danni Zhong Qiaolin Wei Bin Song Wanlin Li Yiling Zhong Houyu Wang Fenglin Dong Min Zhou Yao He 

机构地区：[1]Suzhou Key Laboratory of Nanotechnology and Biomedicine,Jiangsu Key Laboratory for Carbon-Based Functional Materials&Devices,Institute of Functional Nano and Soft Materials(FUNSOM),Soochow University,Suzhou 215123,China [2]School of Public Health,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China [3]Zhejiang University-University of Edinburgh Institute(ZJU-UoE Institute),Zhejiang University,Haining 314400,China [4]Department of Ultrasound,the First Affiliated Hospital of Soochow University,Suzhou 215006,China [5]State Key Laboratory(SKL)of Biobased Transportation Fuel Technology,Zhejiang University,Hangzhou 310027,China [6]School of Pharmacy,Hangzhou Normal University,Hangzhou 311121,China [7]Macao Translational Medicine Center,Macao University of Science and Technology,Taipa 999078,Macao,China [8]Macao Institute of Materials Science and Engineering,Macao University of Science and Technology,Taipa 999078,Macao,China

出　　处：《Nano Research》2024年第8期7404-7414,共11页纳米研究（英文版）

基　　金：the National Natural Science Foundation of China(Nos.21825402,31400860,22393932,T2321005,and 22204117);the Science and Technology Development Fund,Macao SAR(Nos.0002/2022/AKP and 0115/2023/RIA2);the National Key R&D Program of China(No.2023YFB3208200);the Natural Science Foundation of Jiangsu Province of China(Nos.BK20191417 and BK20170061);the Program for Jiangsu Specially Appointed Professors to Y.H.,a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD);111 Project and Collaborative Innovation Center of Suzhou Nano Science and Technology(NANO-CIC).

摘　　要：Despite sufficient studies performed in non-primate animal models,there exists scanty information obtained from pilot trials in non-human primate animal models,severely hindering nanomaterials moving from basic research into clinical practice.We herein present a pioneering demonstration of nanomaterials based optical imaging-guided surgical operation by using macaques as a typical kind of non-human primate-animal models.Typically,taking advantages of strong and stable fluorescence of the small-sized(diameter:~5 nm)silicon-based nanoparticles(SiNPs),lymphatic drainage patterns can be vividly visualized in a real-time manner,and lymph nodes(LN)are able to be sensitively detected and precisely excised from small animal models(e.g.,rats and rabbits)to non-human primate animal models(e.g.,cynomolgus macaque(Macaca fascicularis)and rhesus macaque(Macaca mulatta)).Compared to clinically used invisible near-infrared(NIR)lymphatic tracers(i.e.,indocyanine green(ICG);etc.),we fully indicate that the SiNPs feature unique advantages for naked-eye visible fluorescence-guided surgical operation in long-term manners.Thorough toxicological analysis in macaque models further provides confirming evidence of favorable biocompatibility of the SiNPs probes.We expect that our findings would facilitate the translation of nanomaterials from the laboratory to the clinic,especially in the field of cancer treatment.

关 键 词：fluorescent silicon nanoparticles lymph node REAL-TIME naked-eye visualization macaque models 

分 类 号：O657.3[理学—分析化学]