超声造影定量评价顺铂联合姜黄素对喉咽癌移植瘤抗血管生成作用的价值研究  

Value of quantitative evaluation of contrast-enhanced ultrasound on the anti-angiogenesis by combining cisplatin with curcumin in the treatment of laryngopharyngeal carcinoma xenografts

作　　者：张竣豪 梁丽 刘佳丽 李玲玲 胡巧[3] ZHANG Junhao;LIANG Li;LIU Jiali;LI Lingling;HU Qiao(Guangxi University of Chinese Medicine,Nanning 530200,China;Chenzhou First People’s Hospital of Hunan Province,Chenzhou 423099,China;Department of Ultrasound,the People’s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China)

机构地区：[1]广西中医药大学,南宁530200 [2]湖南省郴州市第一人民医院,郴州423099 [3]广西壮族自治区人民医院超声科,南宁530021

出　　处：《广西医科大学学报》2024年第6期878-883,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.82160339,No.81660292);广西自然科学基金资助项目(No.2024GXNSFAA010381,No.2020GXNSFAA259014)。

摘　　要：目的:探讨超声造影(CEUS)定量分析评价顺铂联合姜黄素治疗裸鼠喉咽癌移植瘤的化疗效果及对血管生成作用的应用价值。方法:建立人喉咽癌FaDu细胞株裸鼠皮下移植瘤模型,随机分为生理盐水组、羧甲基纤维素钠(CMC-Na)组、顺铂组、姜黄素组、顺铂联合姜黄素组(联合组)。比较给药前、后各组肿瘤大小,计算抑瘤率,CEUS定量分析获取峰值强度(PI)、曲线下面积(AUC)、达峰时间(TTP)等血流灌注参数,免疫组织化学染色检测肿瘤微血管密度(MVD)。采用Pearson相关分析法分析CEUS定量参数与MVD及移植瘤体积的相关性。结果:顺铂组、姜黄素组、联合组移植瘤体积较生理盐水组和CMC-Na组缩小(P<0.05),且联合组移植瘤体积最小(P<0.05);顺铂组、姜黄素组、联合组抑瘤率分别为43.62%、37.28%、65.42%。顺铂组、姜黄素组、联合组CEUS定量参数PI、AUC低于生理盐水组和CMC-Na组(P<0.05);顺铂组、姜黄素组、联合组MVD低于生理盐水组和CMC-Na组(P<0.05),且联合组MVD最低(P<0.05)。MVD与PI、AUC、肿瘤体积均呈正相关关系(r=0.937、0.931、0.812,均P<0.05),PI、AUC与肿瘤体积呈正相关系统(r=0.807、0.795,P<0.001)。结论:顺铂联合姜黄素可增强对人喉咽癌裸鼠移植瘤的生长抑制作用;CEUS定量参数PI、AUC可反映肿瘤微血管生成,并能有效评价喉咽癌移植瘤的化疗效果。Objective:To explore the application value of contrast-enhanced ultrasound(CEUS)quantitative analysis in evaluating the chemotherapeutic effect and anti-angiogenesis influence of cisplatin combined with curcumin in nude mice of laryngopharyngeal carcinoma xenograft model.Methods:The nude mouse xenograft models of human laryngopharyngeal carcinoma FaDu cell line were established and randomly divided into normal saline group,sodium carboxymethyl cellulose(CMC-Na)group,cisplatin group,curcumin group and cisplatin combined with curcumin group(combined group).Tumor size before and after administration was compared,the tumor inhibition rate was calculated,CEUS and quantitative analysis were conducted to obtain blood perfusion parameters,including peak intensity(PI),area under the curve(AUC),and time to peak(TTP).The tumor microvessel density(MVD)was detected by immunohistochemical staining,and the correlation between CEUS quantitative parameters and MVD and tumor volume was analyzed by Pearson correlation analysis.Results:The tumor volume in the cisplatin group,curcumin group and combined group was smaller than that in the normal saline group and CMC-Na group(P<0.05),and the tumor volume in the combined group was the smallest(P<0.05).The tumor inhibition rates of cisplatin group,curcumin group and combined group were 43.62%,37.28%and 65.42%,respectively.The CEUS quantitative parameters PI and AUC in the cisplatin group,curcumin group and combined group were lower than those in the normal saline group and CMC-Na group(P<0.05).MVD in the cisplatin group,curcumin group and combined group was lower than that in the normal saline group and CMC-Na group(P<0.05),and MVD in the combined group was the lowest(P<0.05).MVD was positively correlated with PI,AUC and tumor volume(r=0.937,0.931,0.812,all P<0.05).PI and AUC were positively correlated with tumor volume(r=0.807,0.795,P<0.05).Conclusion:Cisplatin combined with curcumin can enhance the growth inhibition of human laryngopharyngeal carcinoma xenografts in nude mice.CEUS quan

关 键 词：喉咽癌 超声造影 顺铂 姜黄素 微血管密度 

分 类 号：R445.1[医药卫生—影像医学与核医学]