当归红芪超滤物对电离辐射引起HUVECs氧化应激和炎症损伤的影响及其机制  

Effects of Radix Angelica sinensis and Radix Hedysari ultrafiltration on oxidative stress and inflammatory damage of HUVECs induced by ionizing radiation and its mechanism

作　　者：王丽蓉 王玲云 李晓静 赵会林 霍耀辉 柴辉 刘凯 WANG Li-rong;WANG Ling-yun;LI Xiao-jing;ZHAO Hui-lin;HUO Yao-hui;CHAI Hui;LIU Kai(School of Traditional Chinese and Wetern Medicine,Gansu University of Chinese Medicine,Lanzhou 730000,China;Cardiology Department,Wuwei Hospital of Traditional Chinese medicine hospital,Wuwei Gansu 733000,China;Cardiology Department,The First People's Hospital of Lanzhou,Lanzhou 730000,China;School of Clinical Medicine,Gansu Medical College,Pingliang Gansu 744000,China)

机构地区：[1]甘肃中医药大学中西医结合学院,甘肃兰州730000 [2]武威市中医医院心血管内科,甘肃武威733000 [3]兰州市第一人民医院心血管内科,甘肃兰州730000 [4]甘肃医学院临床医学院,甘肃平凉744000

出　　处：《中国药理学通报》2024年第8期1501-1509,共9页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No.82260869);兰州市人才创新创业项目(No.20219-RC-71)。

摘　　要：目的探讨当归红芪超滤物(Radix Angelica sinensis and Radix Hedysari ultrafiltration,RAS-RH)对电离辐射致人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)氧化应激和炎症损伤的影响。方法6 Gy X射线构建辐射HUVECs损伤模型,用不同浓度(100、200、400μg·L^(-1))RAS-RH作用于HUVECs,采用CCK-8法检测细胞增殖活性,透射电镜观察细胞线粒体的结构变化,DCFH-DA探针法检测细胞内ROS水平,JC-1试剂盒检测细胞内线粒体膜电位变化,流式细胞术检测细胞凋亡和周期,ELISA法检测细胞内IL-6和TNF-α含量,生化试剂盒检测MDA、CAT、SOD及GSH-PX活性,qRT-PCR检测细胞内Nrf2、HO-1、NF-κB、eNOS、IL-6基因表达水平,Western blot法检测细胞内Nrf2、HO-1、eNOS、NF-κB、p-NF-κB和IL-6蛋白的表达水平。结果与模型组相比,给药组HUVECs活性更高,细胞凋亡率下降,细胞内ROS水平降低,细胞内线粒体结构更加完整,线粒体膜电位水平更高,细胞内Nrf2、HO-1和eNOS的表达增加,p-NF-κB、IL-6的表达降低。结论RAS-RH具有抗辐射、抗氧化及抗炎作用,可能通过激活Nrf2/HO-1信号通路活性,降低电离辐射导致的HUVECs氧化应激及炎症损伤,从而促进细胞增殖活性。Aim To investigate the effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration(RAS-RH)on oxidative stress and inflammatory injury of human umbilical vein endothelial cells(HUVECs)induced by ionizing radiation.Methods The model of HUVECs damage induced by 6 Gy X-rays was established.HUVECs were treated with different concentrations of RAS-RH(100,200,400μg·L^(-1)).The proliferative activity of HUVECs was detected by CCK-8 method,the structural changes of mitochondria were observed by transmission electron microscope,the level of ROS was detected by DCFH-DA probe,the change of intracellular mitochondrial membrane potential was detected by JC-1 kit,and the apoptosis and cycle were detected by flow cytometry.The contents of IL-6 and TNF-αin cells were detected by ELISA.The activities of MDA,CAT,SOD and GSH-PX were detected by biochemical kit.The gene expression levels of Nrf2,HO-1,NF-κB,eNOS and IL-6 were detected by qRT-PCR,and the expression levels of Nrf2,HO-1,eNOS,NF-κB,p-NF-κB and IL-6 protein were detected by Western blot.Results Compared with the model group,RAS-RH could increase the activity of HUVECs induced by ionizing radiation,decrease the rate of apoptosis,decrease the level of intracellular ROS,reduce the injury of intracellular mitochondria,increase the level of mitochondrial membrane potential,promote the expression of Nrf2,HO-1 and eNOS,and inhibit the expression of NF-κB and IL-6.Conclusions RAS-RH has anti-radiation,antioxidant and anti-inflammatory effects,which may reduce the oxidative stress and inflammatory damage of HUVECs induced by ionizing radiation by activating the activity of Nrf2/HO-1 signal pathway,thus promoting the activity of cell proliferation.

关 键 词：电离辐射 当归红芪超滤物 脐静脉血管内皮细胞 氧化应激 炎症损伤 Nrf2/HO-1信号通路 

分 类 号：R282.71[医药卫生—中药学] R322.123[医药卫生—中医学] R329.24R349.1R364.5R818