板青颗粒特征图谱及其质量相关性研究  

Characteristic chromatogram and quality correlation of Banqing granules

作　　者：周景瑞 艾蓉 姜玲玲 冉江[1] 罗文菊 张琴 章厉劼 余波 ZHOU Jingrui;AI Rong;JIANG Lingling;RAN Jiang;LUO Wenju;ZHANG Qin;ZHANG Lijie;YU Bo(Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang Guizhou 550000,China;Guizhou Veterinary Medicine and Feed Testing Institute,Guiyang Guizhou 550000,China)

机构地区：[1]贵州省农业科学院畜牧兽医研究所,贵州贵阳550000 [2]贵州省兽药饲料检测所,贵州贵阳550000

出　　处：《中兽医医药杂志》2024年第4期73-77,共5页Journal of Traditional Chinese Veterinary Medicine

基　　金：贵州省科技厅项目(黔科合成果[2021]一般039、黔科合成果[2022]一般008);贵州省农业农村厅项目(GZCYTX-0302)。

摘　　要：本研究旨在开发一种用于检测板青颗粒中靛玉红含量的方法,并进行指纹图谱分析。采用高效液相色谱技术,色谱柱选用Eclipse Plus C_(18)柱(4.6 mm×250.0 mm,5μm),检测波长289 nm,以甲醇-水(77∶23)为流动相,等度洗脱,流速为0.8 mL/min,柱温30℃,进样量10μL。结果显示,在对靛玉红定性分析中线性关系良好(R^(2)=1.0000),平均回收率为98.07%,12批次样品中检测出共有峰7个,相似度为0.791~1.000。该方法操作简单、快捷,结果准确,可用于板青颗粒中靛玉红含量的测定及板青颗粒质量评价。The objective was to develop a method for content determination of indirubin in Banqing granules and conduct fingerprint chromatogram.High performance liquid chromatography was used with Eclipse Plus C_(18)column(4.6 mm×250.0 mm,5μm).Detection wavelength was 289 nm using methanol-water (77∶23) as the mobile phase in equimolar elution.The flow rate was 0.8 mL/min with column temperature of 30℃and injection volume of 10μL.The results showed that in the qualitative analysis of indirubin,the linear relationship was good (R~2=1.000 0),and the average recovery rate was 98.07%.A total of 7 peaks were detected in 12 batches of samples with a similarity of 0.791-1.000.The experimental operation is simple,fast,and the results are accurate.It can be used for the determination of indirubin content in Banqing granules,and for the study of characteristic chromatogram and quality evaluation of Banqing granules.

关 键 词：板青颗粒 靛玉红 质量评价 指纹图谱 

分 类 号：S853.7[农业科学—临床兽医学]