芪红散对巨噬细胞分化及炎症水平的影响  

Efficacy of Qi Hong San on macrophage differentiation and inflammation levels

作　　者：姜海兵[1] 杨毅 翟雪芹[1] 张莉晶 高玉[1] 姜述斌[2] JIANG Haibing;YANG Yi;ZHAI Xueqin;ZHANG Lijing;GAO Yu;JIANG Shubin(Department of Cardiology,the Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University,Urumqi 830000,China;Cardiac Intensive Care Unit/CCU,the Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属中医医院心内科,乌鲁木齐830000 [2]新疆医科大学附属中医医院心脏重症监护室/CCU,乌鲁木齐830000

出　　处：《新疆医科大学学报》2024年第7期1019-1023,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金面上项目(2021D01C229)。

摘　　要：目的 研究芪红散对巨噬细胞分化及炎症水平的影响及其机制。方法 将单核细胞在PKC激活剂(PMA)的诱导下转化为巨噬细胞。仅使用PMA诱导的细胞定义为M0组,使用脂多糖(LPS)和γ干扰素(IFN-γ)诱导处理的细胞定义为M1组,在使用LPS和IFN-γ诱导处理的基础上加入低剂量以及高剂量芪红散进行干预的两组分别定义为M1+芪红散-L组以及M1+芪红散-H组。分别采用细胞计数试剂-8(CCK-8)法、流式细胞术、酶联免疫吸附试验(ELISA)、免疫印迹试验(WB)检测4组细胞的增殖、凋亡、迁移情况和肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、p50、p-p65水平。结果 与M0对比,M1组增殖、迁移、CD86^(+)分化比例、TNF-α、IL-6、p50及p-p65水平显著增加(P均<0.05),CD206^(+)分化比例、IL-10水平显著降低(P均<0.05)。与M1组比较,M1+芪红散-L组与M1+芪红散-H组CD86^(+)分化比例、TNF-α、IL-6表达水平均降低(P<0.05),且M1+芪红散-H组TNF-α及IL-6表达水平明显低于M1+芪红散-L组(P<0.05),IL-10表达水平明显高于M1+芪红散-L组(P<0.05)。与M1组比较,M1+芪红散-H组p50及p-p65表达水平均降低(P<0.05),M1+芪红散-L组206^(+)分化显著升高(P<0.05)。结论 芪红散可有效地减少巨噬细胞的CD86^(+)型分化,抑制炎症信号通路核因子-κB(Nuclearfactor kappaB,NF-κB)的激活从而降低炎症因子的表达,减轻炎症反应。Objective The effects of Qi Hong San on macrophage differentiation and inflammation level and its mechanism were investigated.Methods Monocytes were transformed into macrophages induced by PKC activator( PMA).Cells induced with PMA only were defined as group M0,cells treated with lipopolysaccharide(LPS) and interferon-γ(IFN-γ) were defined as group M1,and the 2 groups intervened with low as well as high doses of Qi Hong San on top of the induction treatments with LPS and IFN-γ,respectively,andwere defined as M1+Qi Hong San-L group and M1+Qi Hong San-H group.The proliferation,apoptosis,migration,and levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10),p50,and p-p65 of the 4 groups of cells were detected using cell counting kit-8(CCK-8),flow cytometry,enzyme-linked immunosorbent assay(ELISA),andWestern Blot(WB).Results Compared with M0,the proliferation,migration,CD86~+ differentiation,TNF-α,IL-6,P50 and P-P65 in M1 group was increased significantly(all P<0.05),while CD206~+ differentiation and Il-10 was decreased significantly(all P<0.05).Compared with M1 group,M1+Qi Hong San-L group and M1+Qi Hong San-H group,CD86~+ differentiation ratio,TNF-α,IL-6 expression levels were decreased(P<0.05).The expression levels of TNF-α and IL-6 in M1+Qi Hong San-H group were significantly lower than those in M1+Qi Hong San-L group(P<0.05),and the expression levels of Il-10 in M1+Qi Hong San-L group were significantly higher than those in M1+Qi Hong San-H group(P<0.05).Compared with M1 Group,the expression levels of P50 and p-p65 in M1+Qi Hong San-L were decreased significantly(P<0.05),the expression levels of CD206~+ differentiation in M1+Qi Hong San-L group were significantly higher(P<0.05).Conclusion Qi Hong San can effectively reduce the CD86~+ differentiation of macrophages,inhibit the activation of the inflammatory signaling pathway NF-κB,there by reducing the expression of inflammatory factors and attenuating the inflammatory response.

关 键 词：芪红散 巨噬细胞 P50 p-p65 炎症 

分 类 号：R289[医药卫生—方剂学]