芪附理中灌肠方对溃疡性结肠炎模型大鼠肠黏膜机械屏障的影响及机制研究  

Study on effects and mechanism of Qifu Lizhong Enema Prescription on mechanical barrier function of intestinal mucosa in rats with ulcerative colitis

作　　者：李微 袁玲玲 李佳欣 韦鹏飞 胡双元 郝彦伟 张怡[1] Wei Li;Lingling Yuan;Jiaxin Li;Pengfei Wei;Shuangyuan Hu;Yanwei Hao;Yi Zhang(Gastroenterology Department,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

机构地区：[1]成都中医药大学附属医院消化内科,成都610075

出　　处：《国际中医中药杂志》2024年第7期874-880,共7页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81973821);成都中医药大学“杏林学者”学科人才研究专项(ZRQN2019008)。

摘　　要：目的观察芪附理中灌肠方对脾肾阳虚型溃疡性结肠炎模型大鼠肠黏膜机械屏障的影响, 探讨其作用机制。方法将70只雄性SD大鼠按随机数字表法分为空白组、模型组、美沙拉嗪组及芪附理中灌肠方高、中、低剂量组, 空白组10只, 其余各组12只。除空白组外, 其余各组采用苦寒泻下法(大黄煎剂)合并三硝基苯磺酸(TNBS)/55%乙醇复合法诱导法制备UC大鼠模型。造模成功后, 空白组和模型组灌肠生理盐水1 ml, 芪附理中灌肠方高、中、低剂量组灌肠芪附理中汤药液3.00、1.50、0.75 g/kg, 美沙拉嗪组灌肠美沙拉嗪溶液0.03 g/kg, 1次/d, 连续干预14 d。14 d后进行疾病活动指数(DAI)评分, 采用HE染色观察结肠病理组织, 免疫组化染色和Western blot法检测结肠组织闭合蛋白(Occludin)及连接黏附分子A(JAM-A)蛋白表达。结果 HE染色结果显示, 模型组黏膜结构破坏, 炎症细胞浸润, 可见水肿和溃疡灶;美沙拉嗪组及芪附理中灌肠方各剂量组黏膜结构较完整, 可见新生上皮炎症浸润、水肿、溃疡均有好转。与模型组比较, 芪附理中灌肠方各剂量组DAI评分降低(P<0.01), 芪附理中灌肠方高、中剂量组结肠组织Occludin、JAM-A蛋白表达升高(P<0.05)。结论芪附理中灌肠方可缓解UC大鼠症状及病理形态, 其修复肠黏膜屏障的作用机制可能与上调Occludin、JAM-A蛋白表达有关。Objective To observe the effects of Qifu Lizhong Enema Prescription on ulcerative colitis rats with yang deficiency of spleen and kidney syndrome;To discuss its mechanism.Methods Totally 70 male SD rats were randomly divided into blank group,model group,mesalazine group,Qifu Lizhong Guanchang Prescription high-,medium-and low-dosage groups;blank group(n=10),other groups(n=12).Except for the blank group,the other groups used bitter cold purgative therapy(Dahuang Decoction)by gavage,and combined with trinitrobenzen sulfonic acid(TNBS)+55%ethanol compound method to induce UC rat model.After successful modeling,the blank group and model group were given 1 ml normal saline enema daily,Qifu Lizhong Enema Prescription groups were given Qifu Lizhong Enema Prescription 3.00,1.50,0.75 g/kg enema daily,and the mesalazine group was given mesalazine 0.03 g/kg enema daily,once a day for consecutive 14 days.After 14 days,Disease Activity Index(DAI)score was performed,and hematoxylin-eosin staining(HE)was used to observe the pathological tissues of the colon.The expressions of Occludin and adhesion molecules A(JAM-A)protein in colon tissue were detected by immunohistochemistry and Western blot.Results HE results showed that the mucosal structure was damaged,inflammatory cells were infiltrated,edema and ulcer foci were observed in model group.The mucosal structure of mesalazine group and Qifu Lizhong Enema Prescription groups were intact,and inflammatory infiltration,edema and ulcer of neoepithelial were improved.Compared with model group,the DAI scores of Qifu Lizhong Enema Prescription groups decreased(P<0.01),the expressions of Occludin and JAM-A in Qifu Lizhong Guanchang Prescription high-and medium-dosage groups significantly increased(P<0.05).Conclusion Qifu Lizhong Enema Prescription can significantly relieve the symptoms and pathological morphology of UC rats,and the mechanism of repairing intestinal mucosal barrier may be related to up-regulating the expressions of Occludin and JAM-A proteins.

关 键 词：结肠炎 溃疡性 芪附理中灌肠方 紧密连接部 连接黏附分子A 大鼠 

分 类 号：R285.5[医药卫生—中药学]