健骨颗粒正丁醇极性部位对去卵巢大鼠OPG/RANKL的影响  

Effects of Jiangu Granules n-butanol polar fraction on OPG/RANKL in ovariectomized rats

作　　者：王志强 周芬 陈翔 黄云梅 魏振朴 孙攀[1,4] 林燕萍 WANG Zhiqiang;ZHOU Fen;CHEN Xiang;HUANG Yunmei;WEI Zhenpu;SUN Pan;LIN Yanping(Institute of Integrated Traditional Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;College of Acupuncture and Massage,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Fujian Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Senile Diseases,Fuzhou 350122,China;Traditional Chinese Medicine Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]福建中医药大学中西医结合研究院,福州350122 [2]福建中医药大学针灸推拿学院,福州350122 [3]福建省中西医结合老年性疾病重点实验室,福州350122 [4]上海中医药大学附属市中医医院,上海200071

出　　处：《中华中医药杂志》2024年第7期3724-3728,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省财政专项(No.X2019002-财政专项);福建省自然科学基金项目(No.2020J01746)。

摘　　要：目的:观察健骨颗粒正丁醇极性部位对去卵巢大鼠骨保护素(OPG)和核因子-κB配体受体致活剂(RANKL)的影响,探讨其治疗绝经后骨疏松症(PMOP)的作用机制。方法:切除雌性SD大鼠双侧卵巢制造PMOP模型,运用双能X线骨密度仪测定骨密度(BMD);ELISA法检测血清中雌二醇(E_(2))、抗酒石酸酸性磷酸酶(TRAP)、OPG和RANKL的含量,并计算OPG/RANKL比值;Western Blot法测定骨组织中TRAP、OPG和RANKL蛋白表达,并计算OPG/RANKL比值。结果:与生理盐水组比较,正丁醇组术后第12周BMD显著增高(P<0.01),术后第8、12周血清E_(2)和OPG水平显著升高(P<0.01);术后8周TRAP在血清中的含量和在骨组织中的蛋白表达均显著降低(P<0.01);术后第8周骨组织OPG蛋白表达显著升高(P<0.01);术后第4、8周骨组织中RANKL蛋白表达均显著降低(P<0.01);术后3个时间点血清中OPG/RANKL比值都显著升高(P<0.01)。结论:健骨颗粒正丁醇极性部位可调节OPG与RANKL之间的代谢平衡,提高血清中OPG/RANKL的比值,降低骨吸收,改善去卵巢大鼠骨骼的代谢水平,提高BMD。Objective:To observe the effect of Jiangu Granules n-butanol polar fraction on osteoprotegerin(OPG)and receptor activator of nuclear factor-κB ligand(RANKL)in ovariectomized rats,and to explore its mechanism in the treatment of postmenopausal osteoporosis(PMOP).Methods:The model was established by ovariectomy in female SD rats.Bone mineral density(BMD)was measured by dual energy X-ray absorptiometry.E_(2)TRAP,OPG,RANKL were detected by ELISA,and calculated the OPG/RANKL ratio.The contents of TRAP,OPG and RANKL proteins in bone tissue were measured by Western Blot,and calculated the OPG/RANKL ratio.Results:Compared with normal saline group,BMD in n-butanol group at 12 weeks after surgery was significantly increased(P<0.01),serum E_(2)and OPG levels at 8 and 12 weeks after surgery were significantly increased(P<0.01);the content of TRAP in serum and protein expression in bone tissue were significantly decreased at 8 weeks after operation(P<0.01);the expression of OPG protein in bone tissue was significantly increased at 8 weeks after operation(P<0.01);the expression of RANKL protein in bone tissue was significantly decreased at 4 and 8 weeks after operation(P<0.01);the OPG/RANKL ratio in serum was significantly increased at three time points after operation(P<0.01).Conclusion:The n-butanol polar fraction of Jiangu Granules can regulate the metabolic balance between OPG and RANKL,increase the ratio of OPG/RANKL in serum,reduce bone resorption,improve the metabolic level of rat bone after oophorectomy and increase BMD.

关 键 词：健骨颗粒 正丁醇极性部位 去卵巢大鼠 骨代谢 绝经后骨质疏松症 骨保护素 核因子-κB配体受体致活剂 机制 

分 类 号：R285.5[医药卫生—中药学]