基于促炎因子探讨益肾通络方对单侧输尿管结扎大鼠肾功能的干预作用  

Intervention of kidney function of unilateral ureteral obstruction rats by Yishen Tongluo Formula based on proinflammatory factors

作　　者：赵盈坤 刘兰英 徐书 杨蕾 刘烨 田哲菁 姚雯静 闫英睿 ZHAO Yingkun;LIU Lanying;XU Shu;YANG Lei;LIU Ye;TIAN Zhejing;YAOWenjing;YAN Yingrui(Beijing University of Chinese Medicine,Beijing 100029,China;Nephrology Department,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学第三附属医院肾病科,北京100029

出　　处：《中华中医药杂志》2024年第7期3734-3737,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京中医药大学新教师项目(No.2021-JYB-XJSJJ-085)。

摘　　要：目的:从促炎因子角度探讨益肾通络方对单侧输尿管结扎(UUO)模型大鼠肾功能的改善作用。方法:将30只雄性大鼠随机分为正常组、假手术组、UUO模型组、贝那普利组和益肾通络方组,每组6只。正常组、假手术组及UUO模型组大鼠每日予10mL/kg蒸馏水灌胃,贝那普利组每日予盐酸贝那普利片1.0mg/kg灌胃,益肾通络方组每日予益肾通络方14.6g/kg灌胃,各组连续给药干预4周。用ELISA法测定各组大鼠血清中白细胞介素(IL)-1β、IL-18、单核细胞趋化因子-1(MCP-1)、肿瘤坏死因子-α(TNF-α)和α1-微球蛋白的表达水平,用生化仪测定血清中尿素氮、肌酐、尿酸、24h尿蛋白定量的含量。结果:与UUO模型组比较,益肾通络方组各项数值均显著降低(P<0.05),益肾通络方组在尿素氮、肌酐、尿酸、α1-微球蛋白和24h尿蛋白定量方面较贝那普利组降低更显著(P<0.05),且对MCP-1的改善作用优于贝那普利组(P<0.05)。结论:益肾通络方可通过下调促炎因子缓解局部炎症反应、改善肾功能和延缓肾病进程.Objective:To research the effect of Yishen Tongluo Formula on the kidney function of unilateral ureteral obstruction(UUO)rats from the angle of proinflammatory factors.Methods:Thirty male rats were divided into normal group,sham operation group,UUO group,Benazepril group and Yishen Tongluo Formula group,randomly,with 6 rats in each group.The normal group,sham operation group and UUO group were given 10 mL/kg deionized water every day,the Benazepril group was given Benazepril Hydrochloride Tablets 1.0 mg/kg every day,the Yishen Tongluo Formula group was given 14.6 g/kg traditional Chinese medicine every day.Each group was dosed continuously for 4 weeks.The expression of IL-1β,IL-18,MCP-1,TNF-αandα1-microglobulin in serum of rats in each group could be determined by ELLSA.Urea nitrogen,uric acid,creatinine in serum and 24-hour urine protein were determined by biochemical analyzer.Results:Compared with the UUO group,the various indicators values of Yishen Tongluo Formula group decreased(P<0.05),Yishen Tongluo Formula group showed changes in urea nitrogen,creatinine,uric acid,α1-microglobulin and 24-hour urine protein decreased more significantly compared to the Benazepril group(P<0.05),and the improvement of MCP-1 was better than that of the Benazepril group(P<0.05).Conclusion:Yishen Tongluo Formula can relieve the proinflammatory factors,improve the renal function and delay the development process of nephropathy by down-regulate the expressions of inflammatory factors.

关 键 词：益肾通络方 单侧输尿管结扎模型 促炎因子 肾功能 肾间质纤维化 机制 

分 类 号：R285.5[医药卫生—中药学]