JS-K对HBV阳性肝癌鸡胚移植瘤生长影响的实验研究  

Experimental study on the impact of JS-K on the growth of HBV-positive HCC in transplanted chicken embryo tumors

作　　者：廖晏蔺 刘正芸 文娟 陈国丽 于万 王欢 LIAO Yan-lin;LIU Zheng-yun;WEN Juan;CHEN Guo-li;YU Wan;WANG Huan(Key Laboratory of Infectious Disease&Biosafety,Provincial Department of Education;School of Basic Medicine,Zunyi Medical University,Zunyi 563000,Guizhou,China)

机构地区：[1]遵义医科大学贵州省普通高等学校传染病与生物安全特色重点实验室,贵州遵义563000 [2]遵义医科大学基础医学院,贵州遵义563000

出　　处：《川北医学院学报》2024年第8期1014-1017,共4页Journal of North Sichuan Medical College

基　　金：贵州省科技厅资助项目[黔科合基础(2020)1Y349];贵州省教育厅资助项目[黔教技(2022)027号]。

摘　　要：目的:在乙型肝炎病毒(HBV)阳性肝癌鸡胚移植模型中,探讨一氧化氮供体JS-K对肿瘤细胞生长的影响。方法:将HBV阳性肝癌细胞HepG2.2.15接种于9日龄受精鸡胚中,按照处理方式不同分为5组:阴性对照组,JS-K低、中、高剂量组(1、5、10μg/个),索拉非尼(Sora)组(400μg/个),每组各3只。2 d后按分组给药,给药采用直接滴加于移植瘤上的方式,1次/d,连续8 d。实验结束后取出鸡胚移植瘤,观察各组移植瘤大小,拍照并称重,采用HE染色法观察各组JS-K对肿瘤细胞的影响;采用免疫印迹法检测肿瘤组织中迁移相关蛋白Vimentin及N-Cadherin的表达情况。结果:与对照组相比,不同剂量JS-K作用后,鸡胚移植瘤体积均明显减小,其中JS-K高剂量组肿瘤体积最小(P<0.05);HE染色可见JS-K作用后瘤细胞数量明显减少;JS-K低剂量组鸡胚移植瘤波形蛋白Vimentin表达水平高于其他组,N-cadherin表达水平低于其他组(P<0.05)。结论:JS-K对HBV阳性肝癌鸡胚移植瘤具有抑瘤作用,该效应可能与抑制鸡胚移植瘤细胞迁移有关。Objective:To explore the impact of the nitric oxide donor JS-K on tumor cell growth within a hepatitis B virus(HBV)-positive liver cancer model using chicken embryo transplantation.Methods:HepG2.2.15 cells,an HBV-positive liver cancer cell line,were inoculated into fertilized chicken embryos at 9 days of age.2 days post-inoculation,treatments were initiated,dividing the experiment into five groups:control group,JS-K 1μg group,JS-K 5μg group,JS-K 10μg group,and sorafenib group(400μg/piece)according to different processing methods,3 subjects in each group.Treatments were administered directly onto the tumors daily for 8 consecutive days,once a day.Post-treatment,tumors were excised for analysis.The size of each tumor was measured,photographed,and weighed,and the tumor inhibition rate was calculated.The effect of JS-K on tumor cells was evaluated using hematoxylin and eosin(HE)staining,and the expression of the cell migration-related protein Vimentin and N-Cadherin in tumor tissue were analyzed by Western blotting.Results:Compared with the control group,tumor volumes in the chicken embryos were reduced following treatment with varying doses of JS-K,with the 10μg group showing the smallest tumor size.HE staining indicated a significant reduction in tumor cell counts post JS-K treatment,the expression level of Vimentin in the 1μg JS-K group of chicken embryo transplant tumors was higher than that of the other groups,while the expression of N-cadherin was lower than that of the other groups.Conclusion:JS-K demonstrates a tumor-suppressive effect in HBV-positive liver cancer in a chicken embryo transplant model,which may be related to inhibiting the migration of tumor cells.

关 键 词：乙型肝炎病毒 肝癌 JS-K 鸡胚 肿瘤迁移 

分 类 号：R373.2[医药卫生—病原生物学]