郑怀贤经验方对股骨骨不连大鼠骨折恢复的影响  

Effects of Zheng Huaixian's empirical formula on the recovery of femoral bone nonunion in rats

作　　者：魏林 檀亚军[2] 孙玉 王金涛 叶锐彬[1] WEI Lin;TAN Ya-jun;SUN Yu;WANG Jin-tao;YE Rui-bin(Department of Fascial Injury,Chengdu 610000,Sichuan,China;Department of Orthopedics,Affiliated Sport Hospital of Chengdu Sport University,Chengdu 610000,Sichuan,China;Chengdu Sport University,Chengdu 610000,Sichuan,China)

机构地区：[1]成都体育学院附属体育医院筋伤科,四川成都610000 [2]成都体育学院附属体育医院门诊骨科,四川成都610000 [3]成都体育学院附属体育医院成都体育学院,四川成都610000

出　　处：《川北医学院学报》2024年第8期1018-1021,1035,共5页Journal of North Sichuan Medical College

基　　金：四川省中医药管理局科学技术研究专项(2021MS245)。

摘　　要：目的:探讨郑怀贤经验方对股骨骨不连大鼠骨折恢复的影响。方法:选取36只Wistar大鼠作为研究对象,按照处理方式不同分为空白组、模型组及实验组。模型组和实验组大鼠手术离断股骨干构建股骨骨不连大鼠模型,术后8周;实验组给予郑怀贤经验方(2.7 g/kg)外敷患肢,干预8周;模型组和空白组不做任何处理。观察并比较各组大鼠活动、毛发色泽变化和股骨干缺损、病理改变、血清学指标变化。结果:空白组大鼠活跃,食欲正常、被毛致密紧披有光泽色泽,股骨干骨质结构正常、软骨细胞排列有序;模型组大鼠活动状态减少、食欲降低、毛发稍燥、色泽较暗,且实验组大鼠上述情况有所改善;实验组大鼠干预4周时股骨缺损部位有骨痂,干预8周时骨缺损线消失、骨皮质连续,且与模型组大鼠相比,软骨细胞排列紊乱、结构缺少病理改变有改善,血清骨特异性碱性磷酸酶(BALP)、骨钙素(OC)、I型原胶原N-端前肽(PINP)、转化生长因子-β(TGF-β)、血管内皮细胞生长因子(VEGF)、骨形态发生蛋白-2(BMP-2)水平均高于模型组(P<0.05)。结论:郑怀贤经验方可提高成骨细胞活性、TGF-β、VEGF、BMP水平,促进股骨骨不连大鼠骨折恢复。Objective:To explore the effects of Zheng Huaixian's empirical formula on the recovery of femoral bone nonunion in rats.Methods:The femoral shafts were surgically severed to construct models of rat with femoral bone nonunion,and they were divided into model group and experimental group according to different processing methods.The healthy rats were enrolled as blank group.At 8 weeks after surgery,experimental group was given Zheng Huaixian's empirical formula(2.7 g/kg)for external application on affected limbs for 8 weeks,while model group and blank group were not treated.The changes of rat activities,hair color,defects of femoral shaft,pathological situations and serological indexes were observed.Results:In blank group,rats were active,appetite was normal,clothing hair was compact and glossy,bone structure of femoral shaft was normal,and arrangement of chondrocytes was ordered.Compared with model group,reduced activity state,appetite,hair dryness and dark color were improved in experimental group.There was bone callus at femoral defect site after 4 weeks of intervention,bone defect line was disappeared,bone cortex was continuous,arrangement disorder and structure lack of chondrocytes were improved after 8 weeks of intervention in experimental group.The levels of serum bone alkaline phosphatase(BALP),osteocalcin(OC),type I procollagen N-terminal propeptide(PINP),transforming growth factor-β(TGF-β),serum vascular endothelial growth factor(VEGF)and bone morphogenetic protein-2(BMP-2)in experimental group were higher than those in model group(P<0.05).Conclusion:Zheng Huaixian's empirical formula can improve osteoblasts activity,increase levels of TGF-β,VEGF and BMP,and promote fracture recovery in rats with femoral bone nonunion.

关 键 词：骨不连 郑怀贤经验方 成骨细胞活性 转化生长因子β 血管内皮细胞生长因子 骨形态发生蛋白2 

分 类 号：R687.3[医药卫生—骨科学] R285.5[医药卫生—外科学]