连作对甜瓜根际土壤细菌多样性及群落结构的影响  

Effects of Continuous Cropping on Melon Rhizosphere Soil Bacterial Diversity and Community Structure

作　　者：吴秋芳[1] 何玲敏 张美玲 郜新强 王景顺[1] 齐英 WU Qufang;HE Lingmin;ZHANG Meiling;GAO Xinqiang;WANG Jingshun;QI Ying(College of Biology and Food Engineering,Anyang Institute of Technology,Anyang 455000,China;Anyang Agricultural Ecology and Resource Protection Station,Anyang 455000,China)

机构地区：[1]安阳工学院生物与食品工程学院,河南安阳455000 [2]安阳市农业生态与资源保护站,河南安阳455000

出　　处：《安阳工学院学报》2024年第4期120-128,共9页Journal of Anyang Institute of Technology

基　　金：安阳市重大专项(2021A01NY009);安阳工学院博士基金项目(BSJ2020003)。

摘　　要：通过高通量测序研究不同连作年限甜瓜根际土壤中细菌的多样性和菌群结构,以期了解甜瓜连作的微生态机制,为连作障碍提供防治参考。研究结果表明:随着连作时间的增加,细菌多样性指数和丰富度指数先增加后下降。从4份甜瓜土壤样本中共获得35门和60属的细菌。优势门有6个,分别为:变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)、芽单胞菌门(Gemmatimonadota)、放线菌门(Actinobacteria)、unclassified-Bacteria和拟杆菌门(Bacteroidota)。其中,变形菌门和拟杆菌门的丰度是先增加后下降,放线菌门丰度呈逐渐下降的趋势,酸杆菌门、芽单胞菌门和unclassified-Bacteria和绿弯菌门丰度呈先下降后上升的趋势;优势属分别为unclassified-Gemmatimonadaceae、unclassified-Bacteria、unclassifiedVicinamibacteraceae、unclassified-Vicinamibacterales、Subgroup-10、溶杆菌属(Lysobacter)、鞘脂单胞菌属(Sphingomonas)、假单胞菌属(Pseudomonas)、硝化螺旋菌(Nitrospira)。This study aims to understand the microecological mechanism of continuous cropping of melons and provide reference for preventing and managing continuous cropping obstacles.High-throughput sequencing was used to study the bacterial diversity and community structure in the rhizosphere soil of melons with different continuous cropping years.The results showed that with the increasing duration of continuous cropping,the bacterial diversity index and richness index first increased and then decreased.A total of 35 phyla and 60 genera of bacteria were obtained from the four melon soil samples.There were six dominant phyla:Proteobacteria,Acidobacteriota,Gemmatimonadota,Actinobacteria,unclassified Bacteria and Bacteroidota.Among them,the abundance of Proteobacteria and Bacteroidetes first increased and then decreased,the abundance of Actinobacteria showed a gradual decreasing trend,and the abundance of Acidobacteria,Gemmonadas,unclassified-Bacteria and Chloroflexi first decreased and then increased.The dominant genera were unclassified Gemmatimonadaceae,unclassified-Bacteria,unclassified-Vicinamibacteraceae,unclassified-Vicinamibacterales,Subgroup-10,Lysobacter,Sphingomonas,Pseudomonas and Nitrospira.

关 键 词：高通量测序技术 甜瓜 连作 细菌群落结构 细菌多样性 

分 类 号：S154.36[农业科学—土壤学]