根内根孢囊霉对正茬和迎茬大豆生物量的影响  

Effects of Rhizophagus intraradices on Biomass of Rotational Cropping and Alternate Cropping Soybean

作　　者：张敏 接伟光[1] 谭义雯 阚连宝 孟剑侠[1] 孙珊珊 张勇[1] 石贺 张思怡 ZHANG Min;JIE Weiguang;TAN Yiwen;KAN Lianbao;MENG Jianxia;SUN Shanshan;ZHANG Yong;SHI He;ZHANG Siyi(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education/Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region/Key Laboratory of Microbiology,College of Heilongjiang Province/School of Life Sciences,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学,生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,哈尔滨150080

出　　处：《中国农学通报》2024年第20期37-45,共9页Chinese Agricultural Science Bulletin

基　　金：黑龙江省自然科学基金联合引导项目“根内根孢囊霉与大豆及其根际促生菌的互作机制研究”(LH2023C087);黑龙江省生态环境保护科研项目“大豆根际促生菌的筛选及其降解土壤农药残留研究”(HST2022TR001);黑龙江省省属高等学校基本科研业务费科研项目“丛枝菌根真菌与大豆根际促生微生物的互作机制”(2022-KYYWF-1119);哈尔滨市科技计划自筹经费项目“AM真菌与大豆根际促生菌对大豆抗病性基因表达的影响”(ZC2022ZJ002008)。

摘　　要：本研究旨在探究根内根孢囊霉(Rhizophagus intraradices)在缓解大豆迎茬障碍和提高大豆生物量方面的效应。以‘黑农48’品种的大豆为对象,通过湿筛倾析-蔗糖离心法、碱解离-酸性品红染色法以及直接测量法,分别对AM真菌孢子密度、侵染率及大豆生物量进行了测定。结果显示,正茬大豆田的根际土壤中孢子密度是迎茬的2.2倍。在自然土壤条件下,接种R.intraradices的正茬与迎茬土壤中的大豆植株,其根系AM真菌侵染率和生物量均显著高于未接种处理组,其中根长最为显著,分别提高了31.60%和33.57%。在迎茬土壤中接种R.intraradices菌剂的大豆生物量与正茬土壤中未接种处理组的接近。在相同接菌处理条件下,灭菌土壤中的大豆植株根系AM真菌侵染率和生物量均低于自然土壤。本研究这些发现为缓解大豆迎茬障碍和提升大豆产量提供了重要的理论依据。The purpose of this study was to investigate the role of Rhizophagus intraradices in alleviating alternate cropping resistance and increasing soybean biomass.The soybean variety‘Heinong 48’was used as experimental material.The spore density of arbuscular mycorrhizal(AM)fungi,the colonization rate of AM fungi,and the soybean biomass were determined using wet sieve decantation-sucrose centrifugation,alkali separation-acid fuchsin method and direct measurement,respectively.The results showed that the AM fungi spore density in the rhizosphere soil of rotational cropping soybean field was 2.2 times higher than that of alternate cropping soybean field.Under natural soil conditions,the AM fungal colonization rate of soybean roots and soybean biomass significantly increased after inoculation with R.intraradices in the rotational cropping and alternate cropping soil compared to the untreated group.Additionally,the root length was the most significantly increased by 31.60% and 33.57%,respectively.The soybean biomass inoculated with R.intraradices in the rotational cropping soil was similar to that of the untreated group in the alternate cropping soil.Under the same inoculation conditions,the AM fungal colonization rate of soybean roots and the soybean biomass in the sterilized soil were lower than those in the natural soil.This study laid a theoretical foundation for alleviating the obstacle of soybean alternate cropping resistance and improving soybean yield.

关 键 词：大豆 正茬与迎茬 迎茬障碍 根内根孢囊霉 侵染率 生物量 AM真菌孢子密度 

分 类 号：S529[农业科学—作物学]