6个抗稻瘟病基因在云南省勐海县水稻主栽品种中的分布  

Distribution of Six Rice Blast Resistance Genes in Main Rice Varieties in Menghai County of Yunnan Province

作　　者：田维逵 董丽英[2] 资鸿强 刘树芳[2] 刘沛 杨勤忠[2] TIAN Weikui;DONG Liying;ZI Hongqiang;LIU Shufang;LIU Pei;YANG Qinzhong(Menghai Plant Protection Quarantine and Soil Fertilizer Workstation,Jinghong,Yunnan 666200;Institute of Agricultural Environment and Resource Research,Yunnan Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pest of Yunnan Province,Kunming 650205)

机构地区：[1]云南省勐海县植保植检与土壤肥料工作站,云南景洪666200 [2]云南省农业科学院农业环境资源研究所/云南省农业跨境有害生物绿色防控重点实验室,昆明650205

出　　处：《中国农学通报》2024年第20期130-134,共5页Chinese Agricultural Science Bulletin

基　　金：云南省科技计划项目“云南特色稻麦豆作物重要性状鉴定技术研发与应用”(202302AE090011);云南省农业联合专项“利用2个广谱抗稻瘟病新基因创制水稻抗病新种质”(202301BD070001-115);云南省人力资源与社会保障厅专家基层工作站项目(云人社通[2022]17号)。

摘　　要：为了明确勐海县水稻主栽品种中已克隆的Pi2、Pi9、Piz-t、Pi50/Pigm、Pita-2和Pib等6抗稻瘟病基因的构成。通过对勐海县18个水稻主栽品种为试验材料,利用6个抗病基因的特异分子标记进行抗瘟基因的分子检测。结果显示:抗性基因Pib分布频率最高,检出率为77.78%,Pi2、Pita-2和Piz-t的检出率分别为22.22%、22.22%和11.11%;在供试品种中未检测出抗性基因Pi9和Pi50/Pigm基因。检测到2个抗性基因和1个抗性基因的品种均为8份(均占44.44%),未检测出抗性基因的品种为2份(占11.11%)。结果表明:勐海县水稻主栽品种的抗性遗传背景较为狭窄,需引入新的有效抗源以拓宽水稻品种的抗病性。The purpose of this study is to clarify the composition of the cloned blast resistance genes Pi2,Pi9,Piz-t,Pi50/Pigm,Pita-2,and Pib among the major cultivated rice cultivars in Menghai County.Eighteen major cultivated rice cultivars grown in Menghai County were selected as experimental materials,and molecular detection was carried out using gene-specific molecular markers for the aforementioned 6 resistance genes.The results showed that the distribution frequency of the resistance gene Pib was the highest,with a detection rate of 77.78% among these tested cultivars.The detection rates of Pi2,Pita-2,and Piz-t were 22.22%,22.22%,and 11.11%,respectively.Resistance genes Pi9 and Pi50/Pigm were not detected in the tested cultivars.Among the tested cultivars,there were 8 varieties(each accounting for 44.44%)with 2 resistance genes and 1 resistance gene detected.There was no any one of 6 known resistance genes was detected in 2 cultivars,which was accounting for 11.11%of all tested cultivars.The results indicate that the genetic background of resistance in major rice cultivars cultivated in Menghai County is relatively narrow.It is necessary to introduce new and effective resources of resistance to broaden the resistance spectrum of rice cultivars to rice blast.

关 键 词：水稻稻瘟病 抗性基因 分子标记 勐海县 分子检测 遗传背景 

分 类 号：S4[农业科学—植物保护]