表面增强拉曼散射光谱在miRNAs检测中的研究进展  

Research Progress of Surface-Enhance Raman Scatting Spectrum for miRNAs Detection

作　　者：刘会俏 付金金 王思甜 张家坤 何亚楠 曹康哲 LIU Hui-qiao;FU Jin-jin;WANG Si-tian;ZHANG Jia-un;HE Ya-nan;CAO Kang-zhe(College of Chemistry and Chemical Engineering,Xinyang Normal University,Xinyang 464000,China;Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis,Xinyang 464000,China)

机构地区：[1]信阳师范大学化学化工学院,河南信阳464000 [2]信阳市功能纳米材料生物分析重点实验室,河南信阳464000

出　　处：《光谱学与光谱分析》2024年第8期2111-2119,共9页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(62105277);信阳师范大学“南湖学者奖励计划”青年项目资助。

摘　　要：表面增强拉曼散射(SERS)因其灵敏度高、非侵入性、多路检测等特点,已广泛应用于生物医学检测。研究发现微小核糖核酸(miRNAs)的异常表达与多种疾病有关,miRNAs已成为一种新型的生物标志物。开发简单、灵敏、可靠的miRNAs检测方法,对miRNAs的生物学功能研究、医学诊断、疾病治疗和靶向药物研究具有重要意义。目前,与核酸信号放大策略相结合的纳米SERS探针,用于miRNAs检测表现出较高的灵敏度。然而实际样本中成分复杂,易产生背景信号,干扰检测结果;分离纯化过程繁琐,增加了检测时间。近年来,研究者们通过结合其他技术来优化检测,提高样本检测通量、简化操作、减少分析时间、提高分辨率。该论文主要介绍了基于SERS技术的miRNAs检测方法的最新进展,讨论了技术融合的优势与必要性,并总结了基于SERS技术的miRNAs检测用于临床检测还存在的问题,旨在为设计新型快速灵敏可靠的miRNAs SERS检测平台提供参考。Surface-enhance Raman scatting(SERS)has been widely used in biomedical detection due to its high sensitivity,non-invasive,multi-channel detection and other characteristics.The abnormal expression of microribonucleic acid(miRNAs)has been found to be associated with a variety of diseases,and miRNAs have become a novel biomarker.The development of simple,sensitive and reliable miRNAs detection methods is of great significance for studying biological function,medical diagnosis,disease treatment and targeted drug research of miRNAs.Nano-SERS probes combined with nucleic acid signal amplification strategy showed high sensitivity for miRNAs detection.However,the components in the actual samples are complex,resulting in background signal interference detection results,and the tedious separation and purification process increases the detection time.Researchers have recently combined other techniques to optimize detection,improve sample throughput,simplify operations,reduce analysis time,and improve resolution.This paper mainly introduces the latest progress in miRNAs detection method based on SERS technology,discusses the advantages and necessity of technology fusion,and summarises the existing problems of miRNAs detection based on SERS technology for clinical detection,aiming to provide a reference for the design of a new fast,sensitive and reliable miRNAs detection platform.

关 键 词：拉曼光谱 表面增强拉曼散射 微小核糖核酸 核酸信号放大 

分 类 号：O657.37[理学—分析化学]