HPLC法同时测定知黄抗癌方中7种成分的含量  

Simultaneous content determination of seven constituents in Zhihuang Kang’ai Prescription by HPLC

作　　者：芮黎珺 杨喜梅 王瑶 刘梅[4] 徐嘉 RUI Li-jun;YANG Xi-mei;WANG Yao;LIU Mei;XU Jia(Department of Pharmacy,Fudan University Shanghai Cancer Center Department of Oncology,Shanghai Medical College,Fudan University,Shanghai 200032,China;Shanghai Innovation Center of TCM Health Service,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Assisted Reproduction,Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Technology Transfer Center,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]复旦大学附属肿瘤医院药剂科,复旦大学上海医学院肿瘤学系,上海200032 [2]上海中医药大学中医健康服务协同创新中心,上海201203 [3]上海交通大学医学院附属第九人民医院辅助生殖科,上海200011 [4]上海中医药大学技术转移中心,上海201203

出　　处：《中成药》2024年第7期2153-2156,共4页Chinese Traditional Patent Medicine

基　　金：上海市科委生物医药科技支撑项目(18401931500)。

摘　　要：目的建立HPLC法同时测定知黄抗癌方中红景天苷、绿原酸、芒果苷、虎杖苷、盐酸小檗碱、异牡荆素、白藜芦醇的含量。方法分析采用SPOLARC_(18)色谱柱(4.6mm×250mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0mL/min;柱温30℃;检测波长275nm。结果7种成分在各自范围内线性关系良好(r≥0.9997),平均加样回收率91.32%~106.95%,RSD0.4%~2.9%。结论该方法简便快速,专属性好,可用于知黄抗癌方的质量控制。

关 键 词：知黄抗癌方 化学成分 含量测定 HPLC 

分 类 号：R927.2[医药卫生—药学]