望春花花蕾化学成分及其乙酰胆碱酯酶体外抑制活性研究  

Chemical constituents from the flower buds of Magnolia biondii and their in vitro acetylcholinesterase inhibitory activities

作　　者：曹彦刚 王建超 王梦娜 何玉环 李红伟 郝志友 郑晓珂 冯卫生 CAO Yan-gang;WANG Jian-chao;WANG Meng-na;HE Yu-huan;LI Hong-wei;HAO Zhi-you;ZHENG Xiao-ke;FENG Wei-sheng(Henan Provincial Engineering and Technology Center for Chinese Medicine Development College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南省中药开发工程技术研究中心,河南郑州450046

出　　处：《中成药》2024年第7期2278-2283,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82003946);2016年中央引导地方科技发展专项项目(豫财科[2016]149号)。

摘　　要：目的研究望春花MagnoliabiondiiPamp.花蕾化学成分及其乙酰胆碱酯酶体外抑制活性。方法望春花花蕾50%丙酮提取物采用DiaionHP-20、ToyopearlHW-40C、ODS、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。按课题组前期方法测定化合物的乙酰胆碱酯酶体外抑制活性。结果从中分离得到17个化合物,分别鉴定为crassifolioside(1)、magnolosideB(2)、芦丁(3)、异槲皮苷(4)、槲皮素(5)、northalifoline(6)、cordysininB(7)、脱氧胸腺嘧啶核苷(8)、吲唑(9)、dihydrodehydrodiconiferylalcohol(10)、秦皮乙素(11)、C-藜芦酰乙二醇(12)、3,4-二羟基苯乙醇(13)、3-甲氧基-4-羟基苯乙醇(14)、原儿茶酸(15)、2,4,6-三甲氧基苯酚(16)、丁香酸(17)。结论化合物1~17均为首次从该植物中分离得到,在20μmol/L浓度下均未显示出乙酰胆碱酯酶抑制活性。AIM To study the chemical constituents from flower buds of Magnolia biondii Pamp.and their in vitro acetylcholinesterase inhibitory activities.METHODS The 50%acetone extract from the flower buds of M.biondii was isolated and purified by Diaion HP-20,Toyopearl HW-40C,ODS and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro acetylcholinesterase inhibitory activities of these compounds were determined according to previous method established by research group.RESULTS Seventeen compounds were isolated and identified as crassifolioside(1),magnoloside B(2),rutin(3),isoquercitrin(4),quercetin(5),northalifoline(6),cordysinin B(7),thymidine(8),indazole(9),dihydrodehydrodiconiferyl alcohol(10),aesculetin(11),C-veratroylglycol(12),3,4-dihydroxyphenylethanol(13),3-methoxy-4-hydroxyphenylethanol(14),3,4-dihydroxybenzoic acid(15),2,4,6-trimethoxyphenol(16),syringic acid(17).CONCLUSION Compounds 1-17 are isolated from this plant for the first time,none of which show acetylcholinesterase inhibitory activities at the concentration of 20μmol/L.

关 键 词：望春花 花蕾 化学成分 分离鉴定 乙酰胆碱酯酶 

分 类 号：R284.1[医药卫生—中药学]