丝素蛋白水凝胶中的蛋白构象转变速率对其表面黏附细胞增殖的影响  

Effect of Protein Conformational Transition Rate in Silk Fibroin Hydrogel on Proliferation of Cells Adhering to Its Surface

作　　者：蔡国龙 赵伟焜 祝天浩 姚响 张耀鹏[1] CAI Guolong;ZHAO Weikun;ZHU Tianhao;YAO Xiang;ZHANG Yaopeng(State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,College of Materials Science and Engineering,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学材料科学与工程学院,纤维材料改性国家重点实验室,上海201620

出　　处：《功能高分子学报》2024年第4期312-321,共10页Journal of Functional Polymers

基　　金：国家自然科学基金(52273125,52173031);上海市科学技术委员会资助项目(22520711900)。

摘　　要：以辣根过氧化物酶温和介导交联的丝素蛋白(SF)水凝胶为模型材料,采用改变水凝胶交联密度的策略成功实现对水凝胶中蛋白构象转变速率的有效调控。考察了SF水凝胶中的蛋白构象转变速率对其表面黏附细胞增殖行为的影响。结果表明:水凝胶中的蛋白构象转变速率与交联密度呈反相关关系,即交联密度越小,构象转变越快;水凝胶表面的小鼠胚胎成纤维细胞(NIH3T3)增殖与SF构象转变速率大体呈正相关关系,但综合初始交联密度的影响,最终呈现出中等构象转变速率材料上细胞总量相对较少,最快构象转变速率材料上细胞总量相对较多的趋势。Due to the good extracellular matrix biomimetic properties,silk fibroin(SF)hydrogels have been extensively concerned and studied in the fields of tissue engineering scaffold and 3D cell culture.The most widely used ones among them are mild chemical crosslinked SF hydrogels.However,as the inevitable movement and self-assembly of hydrophobic segments of silk fibroin in such hydrogels,the conformation of silk fibroin transforms from random coil to theβ-sheet conformation with lower energy level,which will dynamically change the basic characteristics(such as the stiffness and pore size)of the hydrogels.Consequently,unknown and non-negligible impacts on the biomedical application of the hydrogel were induced.Based on this perspective,the SF hydrogel,which was crosslinked by horseradish peroxidase in a mild manner,was chosen as model materials.The strategy of changing related crosslinking density was used to successfully regulate the rate of protein conformational transition in SF hydrogels.At the same time,the effect of protein conformational transition rate in such an SF hydrogel on the proliferation of cells adhering to its surface was investigated.Results showed that the rate of protein conformational transition in corresponding hydrogels was inversely related to the crosslinking density.The lower the crosslinking density was,the faster the conformational transition was.Meanwhile,the proliferation of mouse embryonic fibroblasts(NIH3T3)cells on the hydrogel surface was generally positively correlated with the rate of protein conformational transition in corresponding hydrogels.However,as it also existed the effects of the accompanied initial crosslinking densities,the final trend was that the total number of cells on hydrogels with medium conformational transition rate was relatively low,and that on hydrogels with the fastest conformational transition rate was relatively high.

关 键 词：丝素蛋白水凝胶 交联密度 构象转变 细胞增殖 细胞-材料相互作用 

分 类 号：O63[理学—高分子化学]