异丙隆原药对大鼠的致畸作用  

Teratogenicity of isoproturon in rats

作　　者：程秀荣[1] 王海华[1] 陈巍[1] 梁一帆 谢广云[1] CHENG Xiurong;WANG Haihua;CHEN Wei;LIANG Yifan;XIE Guangyun(National Institute of Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《癌变．畸变．突变》2024年第4期294-297,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:研究异丙隆原药对大鼠的致畸作用。方法:将受孕的雌性Wistar大鼠随机分为对照组(花生油)和低(36.9 mg/kg)、中(73.8 mg/kg)、高(369.0 mg/kg)3个异丙隆原药染毒剂量组,每组15只孕鼠。对照组和各染毒组于大鼠妊娠第6~15天灌胃给予受试物,每天1次,连续10 d。于妊娠第20天处死各组孕鼠,观察母鼠和胎鼠的生长、发育情况。结果:与对照组比较,低、中剂量组孕鼠各项观察指标差异均无统计学意义(P>0.05)。高剂量组孕鼠于受孕第9天出现萎靡不振、背毛蓬松、体质量下降(P<0.01);着床数、活胎数减少,死胎数增多,窝均体质量降低(P<0.5);部分胎鼠出现囟门大、枕骨及胸骨骨化不全、肋骨弯曲,骨骼畸形率为27.78%,与对照组相比,差异均具有统计学意义(P<0.05)。结论:在本实验条件下,369.0 mg/kg组异丙隆原药可引起Wistar大鼠的母体毒性和胚胎发育毒性。OBJECTIVE:To investigate teratogenicity of isoproturon in rats.METHODS:Pregnant Wistar rats were divided into 4 groups of 15 rats per group.Control group(peanut oil)and isoproturon(36.9,73.8 and 369.0 mg/kg)were administered by gavage once per day from day 6^(th) to 15^(th) of gestation.Rats were killed at the 20^(th) day of gestation,maternal and embryonic developmental indicators were examined.RESULTS:Compared to the control group,all observation indicators of 36.9 and 73.8 mg/kg of isoproturon group were no statistically significant difference(P>0.05).On the 9th day,the 369.0 mg/kg group showed signs of fatigue,fluffy back hair,and significantly lower body weight(P<0.01).The implantation counts and live fetus counts were lower(P<0.5),dead embryos were increased and the average body weight of the litters were significantly less than that in the control group(P<0.5,P<0.01).Abnormalities were observed such as widened fontanelle,occipital ossifications,and incomplete and rib bending.The skeleton malformation rate of 27.78%was increased compared to the control group(P<0.05).CONCLUSION:Under our conditions,369.0 mg/kg of isoproturon treatment induced maternal toxicity and embryonic developmental toxicity in Wistar rats.

关 键 词：异丙隆原药 WISTAR大鼠 母体毒性 胚胎发育毒性 致畸作用 

分 类 号：R994.4[医药卫生—毒理学]