基于别构转录因子的水中砷离子无细胞传感器  

Cell-free Biosensor Based on Allosteric Transcription Factors for Detecting Arsenic Ions in Aquatic Environment

作　　者：陈淑婷 张永康 康晓丹 赵辰 夏志强 王景峰 谌志强 CHEN Shuting;ZHANG Yongkang;KANG Xiaodan;ZHAO Chen;XIA Zhiqiang;WANG Jingfeng;SHEN Zhiqiang(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Institute of Environmental and Operational Medicine,Academy of Military Medicine,Academy of Military Sciences,Tianjin 300050,China;The 908th Hospital of the Chinese People’sLiberation Army Joint Logistics Support Force,Nanchang 330001,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]军事科学院军事医学研究院环境医学与作业医学研究所,天津300050 [3]中国人民解放军联勤保障部队908医院,江西南昌330001

出　　处：《环境科学与技术》2024年第4期9-15,共7页Environmental Science & Technology

基　　金：军队科研项目(31470234)。

摘　　要：为了探索水环境中砷离子现场快速检测的新方法,文章构建以特异性识别砷的别构转录因子ArsR为识别元件,荧光RNA为输出信号的无细胞生物传感器,实现对砷离子的现场快速检测。结果表明,在最佳实验条件下,建立的无细胞生物传感器可以在1 h内实现对1 nmol/L砷离子的检出,借助酶标仪可以实现在1~1 000 nmol/L浓度范围内的定量检测,线性方程为Y=1 232 lg[As^(3+)]-629.5(R^(2)=0.989 3)。实际水样中砷离子的检测结果与原子吸收光谱法的测量结果基本一致,加标回收率为98.866%~102.001%,且在其他重金属的干扰下也能正确检测目标物。该方法拥有高灵敏度和特异性强的特点,为砷离子的现场快速检测提供了一种新思路。Aiming at developing a novel method for on-site quick detection of arsenic ions in aquatic environment,in this study a cell-free biosensor was designed using ArsR,an allosteric transcription factor that can specifically recognize arsenic,as recognition element with fluorescent RNA as output signal to realize the in-situ detection of arsenic ions.The results obtained with regard to the cell-free biosensor showed that under the optimal experimental condition,the developed biosensor could achieve the detection of 1.0 nmol/L arsenic ions within one hour,and quantitative detection in a linear dynamic range of 1~1000 nmol/L was achieved with the assistance of a microplate reader,the linear equation being Y=1232lg[As^(3+)]-629.5(R^(2)=0.9893).The result of arsenic ion detection using the biosensor basically consistented with the re-sult by use of atomic absorption spectroscopy in the conditon of measuring the water samples of river water,the recovery rate being in the range of 98.866%~102.001%;furthermore,the target arsenic ions could be detected correctly despite the inter-ference of other heavy metal ions.In summary,this method being characterized with high sensitivity and specificity certainly provided a new idea for the rapid on-site detection of arsenic ions.

关 键 词：砷离子 别构转录因子 无细胞传感器 现场检测 

分 类 号：X832[环境科学与工程—环境工程]