成都假单胞菌处理畜禽养殖污水的效果研究  

Study on Effects of Exogenous Addition of Pseudomonas chengduensis on Treatment of Livestock and Poultry Wastewater

作　　者：杨铭晖 郑燕玲 彭焕龙 解开治[1,2] 周超贤 徐培智 顾文杰 卢钰升 石超宏 YANG Minghui;ZHENG Yaning;PENG Huanong;XIE Kaizhi;ZHOU Chaoxian;XU Peizhi;GU Wenjie;LU Yusheng;SHI Chaohong(School of Resources and Environment,Gansu Agricultural University,Lanzhou 730070,China;Key Lab of Nutrient Resource Recycling and Farmland Conservation,Key Lab of Southern Plant Nutrition and Fertilizer of the Ministry of Agriculture and Rural Affairs,Institute of Agricultural Resources and Environment,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Guangdong Agricultural Environment and Farmland Quality Protection Center,Guangzhou 510030,China)

机构地区：[1]甘肃农业大学资源与环境学院,甘肃兰州730070 [2]广东省农业科学院农业资源与环境研究所,农业农村部南方植物营养与肥料重点实验室,广东省养分资源循环利用与耕地保育重点实验室,广东广州510640 [3]广东省农业环境与耕地质量保护中心,广东广州510030

出　　处：《环境科学与技术》2024年第4期71-79,共9页Environmental Science & Technology

基　　金：广州市科技计划项目(2023A04J0986);广东省农业科学院科技人才引进或培养专项资金(R2021YJ-YB1003);广东省农业科学院汕尾分院科技合作专项(2021-分院专项-02);广东省乡村振兴战略专项资金(XM-101022-13,2023TS-3);广东省农业厅现代农业产业体系(2023KJ118);广东省自然科学基金项目(2021A1515011211);广东省外国专家局项目(2019)。

摘　　要：成都假单胞菌WD211是一株从养殖污水中筛选得到的具有良好脱氮性能、良好抗逆性的菌株。文章从畜禽养殖污水原液处理、模拟污水处理和微生物群落结构变化分析等方向对WD211菌污水处理效果进行了初步研究。研究结果表明,外源添加WD211菌对畜禽养殖污水的TN和NH_(4)^(+)-N的去除效率有明显提升,可达25.03%和27.30%,高于空白对照;外源添加WD211菌对畜禽养殖污水的NH_(4)^(+)-N的生化去除能力有促进作用,且这种促进作用在2%添加剂量时更为明显。模拟污水中WD211菌对COD、TN、NH_(4)^(+)-N的去除率分别为83.16%、28.35%、44.58%,仅积累少量的NO_(3)^(-)-N(0.907 mg/L)和NO_(2)^(-)-N(0.047 mg/L)。WD211菌在NH_(4)^(+)-N浓度较高的条件下能发挥较好的性能;外源添加WD211菌后,水样中假单胞菌属、类产碱菌属和副球菌属等脱氮相关菌属丰度上升。Pseudomonas chengduensis WD211,a strain of bacteria screened from aquaculture wastewater,possesses excel-lent performances of denitrification and stress resistance.In this article,a preliminary experiment was conducted to investi-gate the bio-augmentation effectiveness of WD211 in wastewater treatment from the perspectives of livestock and poultry breeding wastewater and simulated wastewater,and further analyze the microbial community structure changes.The findings of the experiment showed that exogenous addition of WD211 bacteria remarkably improved the efficiency of removing TN and NH_(4)^(+)-N from livestock and poultry wastewater:respectively 25.03%and 27.30%higher compared with the blank control;the exogenous addition of WD211 bacteria presented promoting effects on a biochemical NH_(4)^(+)-N removal ability in terms of livestock and poultry wastewater,and the promoting effect was more evident as a adding dosage was 2%.The rates of removing COD,TN and NH_(4)^(+)-N by WD211 bacteria in simulated wastewater were 83.16%,28.35%and 44.58%,respectively,with only a small accumulation of NO_(3)^(-)-N(0.907 mg/L)and NO_(2)^(-)-N(0.047 mg/L),besides WD211 bacteria could play a good performance even under high concentrations of NH_(4)^(+)-N;and in the wake of exogenous addition of WD211 bacteria,the abundance of nitrogen-removal-related bacterial genera such as Pseudomonas,Paenalcaligenes,and Paracoccus in water samples increased.

关 键 词：畜禽养殖污水 成都假单胞菌 生物强化 氨氮 

分 类 号：X703.1[环境科学与工程—环境工程]