玉米南方锈病主效抗病QTL的鉴定和效应分析  

Major QTL Mapping and Effect Analysis for Resistance to Southern Corn Rust

作　　者：李志敏[1] 李政 丁俊强[1] 田志强[1] LI Zhimin;LI Zheng;DING Junqiang;TIAN Zhiqiang(College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学农学院,河南郑州450046

出　　处：《河南农业科学》2024年第7期109-116,共8页Journal of Henan Agricultural Sciences

基　　金：河南省国际合作重点项目(231111520700)。

摘　　要：以玉米南方锈病感病自交系Lx9801为母本、抗病自交系TY4为父本构建了含有165个家系的BC_(1)F_(4)群体,利用23K SNP芯片对亲本和BC_(1)F_(4)群体进行基因型分型,共筛选出4654个亲本间具有多态性的SNP标记,构建高密度的遗传连锁图谱,结合群体在3个环境下的抗性鉴定结果,共检测到6个QTL,可以解释3.93%~17.87%的表型变异。其中位于第6染色体上的qSCR6.01在3个环境中都检测到,可以解释最大17.87%的表型变异,是一个稳定的QTL。利用TY4与Lx9801组配的包含366个家系的BC1F5群体,对qSCR6.01进行精细定位,结合抗病区域内的标记开发和关键重组单株的抗性鉴定,将抗性位点定位在M2与M3标记之间,区间大小为4.09 Mb,此抗病位点暂被命名为RppT。Southern corn rust(SCR)is a major maize disease worldwide.In this study,a BC_(1)F_(4) population including 165 families was derived from the cross between the susceptible line Lx9801 and the resistant line TY4,which was used for dissecting the QTLs for resistance to SCR.A 23K⁃SNP chip was used for genotyping the family lines of the BC_(1)F_(4) population as well as the parental lines.In total 4654 polymorphic SNP markers between the parental lines were selected to construct the high⁃density genetic linkage map.Combined with the phenotype evaluation of resistance to SCR in three environments,a total of six QTLs against SCR were detected,which could explain 3.93%—17.87%of the phenotypic variation.Among these QTLs,one QTL located on chromosome 6(i.e.,qSCR6.01)was a stable QTL in all three environments,which could explain up to 17.87%of the phenotypic variation.The BC1F5 population including 366 families was used for fine⁃mapping qSCR6.01.Combined with marker development in the QTL region and resistance identification of key recombinant plants,the QTL was finally narrowed down to the interval of 4.09 Mb between the markers M3 and M4,and temporarily named as RppT.

关 键 词：玉米 南方锈病 抗病QTL 精细定位 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治] S513[农业科学—植物保护]