荧光磁性碳点对卵巢癌细胞的靶向成像与检测  

Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots

作　　者：钟思意 林小文 刘嘉欣 王如意 梁涛 邓峥峰 钟傲 韩翠平 ZHONG Siyi;LIN Xiaowen;LIU Jiaxin;WANG Ruyi;LIANG Tao;DENG Zhengfeng;ZHONG Ao;HAN Cuiping(School of Medical Imaging,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China;National Demonstration Center for Experimental Teaching Basic Medicine Science Education,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China;Department of Radiology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu 221000,China)

机构地区：[1]徐州医科大学医学影像学院,徐州221004 [2]徐州医科大学基础医学国家级实验教学示范中心,徐州221004 [3]徐州医科大学附属医院影像科,徐州221000

出　　处：《无机化学学报》2024年第8期1483-1490,共8页Chinese Journal of Inorganic Chemistry

基　　金：江苏省高等学校大学生创新创业训练计划项目(No.202010313024Z);江苏省自然科学基金(No.BK20231165)资助。

摘　　要：利用高温碳化法制备了荧光磁性钆掺杂碳点(Gd-CDs),其具有明亮的绿色荧光发射,荧光量子产率为32.6%,T1弛豫效率高达9.02 L·mmol^(-1)·s^(-1)。将Gd-CDs与卵巢癌特异性抗体Anti-HE4连接,得到的Anti-HE4/Gd-CDs纳米探针能对SKOV-3卵巢癌细胞进行特异性的荧光成像和T1加权成像,其荧光信号强度与SKOV-3的细胞浓度成正比,对SKOV-3细胞的检测限为658 cell·mL^(-1)。以该方法检测了血液样品中的SKOV-3细胞。Fluorescent magnetic gadolinium⁃doped carbon dots(Gd⁃CDs)were prepared using a high⁃temperature carbonization method.The prepared Gd⁃CDs emitted bright green fluorescence with a fluorescence quantum yield of 32.6%and a T1 relaxation efficiency of up to 9.02 L·mmol^(-1)·s^(-1).By conjugating with the ovarian cancer⁃specific antibody Anti⁃HE4,the resulting Anti⁃HE4/Gd⁃CDs nanoprobes could perform specific fluorescent imaging and T1⁃weighted imaging of SKOV⁃3 ovarian cancer cells.The fluorescence intensity was proportional to the concentration of SKOV⁃3 cells,with a detection limit of 658 cell·mL^(-1).This method was applied to the detection of SKOV⁃3 cells in blood samples.

关 键 词：钆掺杂碳点 靶向探针 荧光成像 磁共振成像 卵巢癌细胞 

分 类 号：O614.339[理学—无机化学]