板蓝根注射液特征图谱及其质量相关性研究  

Study on the Characteristic Graph and Quality Correlation of Banlangen Injection

作　　者：周景瑞 冉江[1] 姜玲玲 雷露 许浩翔 艾蓉 罗文菊 张琴 章厉劼 余波 ZHOU Jing-rui;RAN Jiang;JIANG Ling-ling;LEI Lu;XU Hao-xiang;AI Rong;LUO Wen-ju;ZHANG Qin;ZHANG Li-jie;YU Bo(Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang 550000,China;Guizhou Veterinary Medicine and Feed Testing Institute,Guiyang 550000,China)

机构地区：[1]贵州省农业科学院畜牧兽医研究所,贵阳550000 [2]贵州省兽药饲料检测所,贵阳550000

出　　处：《中国兽药杂志》2024年第7期42-49,共8页Chinese Journal of Veterinary Drug

基　　金：贵州省科技厅项目“常用中兽药制剂质量控制关键技术示范与推广”(合同编号:黔科合成果[2021]一般039)、“畜禽用抗菌中药复方制剂和饲用天然植物提取物的研究与示范”(合同编号:黔科合成果[2022]一般008);贵州省农业农村厅项目“贵州省现代肉牛产业技术体系建设”(GZCYTX-0302)。

摘　　要：建立板蓝根注射液尿苷、鸟苷、(R,S)-告依春、腺苷含量检测方法及指纹图谱研究。采用高效液相色谱法,色谱柱:Eclipse Plus C18柱(4.6 mm×250 mm,5μm),检测波长250 nm,以甲醇(A)-水(B)为流动相,梯度洗脱(0~3 min,95%B;3~20 min,95%B-90%B;20~40 min,90%B~30%B;40~42 min,30%B~95%B;42~50 min,95%B),流速为1.0 mL/min,柱温箱为30℃,进样量10μL。该实验操作简单、快捷,结果准确,适用板蓝根注射液尿苷、鸟苷、(R,S)-告依春、腺苷含量的测定,建立指纹图谱,对板蓝根注射液进行质量评估。Objective:To establish a method for detecting the content of uridine,guanosine,(R,S)-goitrin,and adenosine in Banlangen injection and to study their fingerprints.High performance liquid chromatography was used,with Eclipse Plus C18 column(4.6 mm×250 mm,5μm)Detection wavelength:250 nm,using methanol(A)-water(B)as the mobile phase,gradient elution(0~3 min,95%B;3~20 min,95%B~90%B;20~40 min,90%B-30%B;40-42 min,30%B~95%B;42~50 min,95%B),flow rate:1.0 mL/min,column temperature chamber:30℃,injection volume:10μL.The experimental operation is simple,fast,and the results are accurate.It is suitable for the determination of uridine,guanosine,(R,S)-goitrin e,and adenosine content in Banlangen injection,and the establishment of a fingerprint for the quality evaluation of Banlangen injection.

关 键 词：板蓝根注射液 尿苷 鸟苷 (R S)-告依春 腺苷 指纹图谱 

分 类 号：S859.79[农业科学—临床兽医学]