不同工艺板青颗粒对脂多糖的体内外降解作用及其诱导的白羽肉仔鸡血清炎症指标的影响  

Effect of Different Processes of Banqing Granules on Anti-inflammatory Performance of White Feather Broilers under Lipopolysaccharide Stress

作　　者：张云 林翠[2,3] 杨平 邵谱[2,3] 张娜 王佳琪[2,3] 孔红君 郝红娜[3] 韩愈杰 ZHANG Yun;LIN Cui;YANG Ping;SHAO Pu;ZHANG Na;WANG Jia-qi;KONG Hong-Jun;HAO Hong-na;HAN Yu-jie(College of Traditional Chinese Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;Hebei Province Research Center of Traditional Veterinary Medicine Engineering Technology,Baoding 071100,China;Baoding Jizhong Pharmaceutical Co.,Ltd.,Baoding 071500,China;China Animal Agricultwe Association,Beijing 100044,China)

机构地区：[1]河北农业大学动物医学院(中兽医)学院,河北保定071000 [2]河北省中兽药技术创新中心,河北保定071500 [3]保定冀中药业有限公司,河北保定071500 [4]中国畜牧业协会,北京100044

出　　处：《中国兽药杂志》2024年第7期58-65,共8页Chinese Journal of Veterinary Drug

摘　　要：为了探讨不同工艺板青颗粒对脂多糖引起白羽肉仔鸡抗炎性能影响,通过测定不同溶媒板青提取液的氨基酸、有机酸、靛玉红及(R,S)-告依春的薄层鉴别,测定(R,S)-告依春、腺苷及尿苷含量判定产品的质量,采用定量显色基质法测定不同溶媒板青提取液体外降解内毒素的作用,同时测定了30%乙醇提取液白羽肉鸡体内降解内毒素和抗炎效果。结果显示,30%乙醇提取板青氨基酸和靛玉红对照品位置斑点大且颜色深,30%乙醇溶媒提取的板青中尿苷含量最高,水提取的板青(R,S)-告依春和腺苷含量高,30%乙醇提取的板青体外降解内毒素效果最好,且体内具有显著降解内毒素效果,同时能抑制脂多糖引起的血清IL-1β、IL-6及TNF-α细胞因子的升高。由此可知,30%乙醇提取板青体内外具有显著降解内毒素效果和抗炎作用。The objective of this study was effect of different processes of Banqing granules on anti-inflammatory performance of white feather broilers under Lipopolysaccharide stress.The thin layer identification of amino acids,organic acids,indirubin and(R,S)-geuichun in the extracts of different solvents was determined.The contents of(R,S)-geuichun,adenosine and uridine were determined to determine the quality of the products.The effects of the extracts of different solvents of geuiqing on endotoxin degradation in vitro were determined by quantitative chromogenic matrix method.At the same time,the effects of 30%ethanol extract on endotoxin degradation and ant-inflammation in white feather broilers were determined.The results showed that the amino acid and indirubin control samples extracted with 30%ethanol had large spots and dark colors.The content of uridine in the indigo extracted with 30%ethanol solvent was the highest,the content of water extracted indigo(R,S)-geuichun and adenosine was the highest.The indigo extracted with 30%ethanol had the best endotoxin degradation effect in vitro,and had a significant internal toxicity degradation effect in vivo.At the same time,It also inhibited the increase of serum IL-1β,IL-6 and TNF-αcytokines induced by lipopolysaccharide.The results indicated that the 30%ethanol extract of Banqing had significant endotoxin degrading and anti-inflammatory effects in vitro and in vivo.

关 键 词：板青颗粒 乙醇 质量评价 内毒素 

分 类 号：S859.79[农业科学—临床兽医学]