海藻糖凝胶对大鼠全层皮肤缺损创面及兔耳瘢痕增生的影响  

Effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears

作　　者：金剑 储云高 Jin Jian;Chu Yungao(Department of Polymer Science,Fudan University,Shanghai 200433,China;Shanghai Medical Device Cosmetics Evaluation and Verification Center Medical Device Evaluation Department 2,Shanghai 201802,China)

机构地区：[1]复旦大学高分子科学系,上海200433 [2]上海市医疗器械化妆品审评核查中心医疗器械审评二部,上海201802

出　　处：《中华烧伤与创面修复杂志》2024年第7期679-688,共10页Chinese Journal of Burns And Wounds

基　　金：上海市“科技创新行动计划”启明星人才项目(23QB1401000)。

摘　　要：目的探讨海藻糖凝胶对大鼠全层皮肤缺损创面及兔耳瘢痕增生的影响。方法该研究为实验研究。制备海藻糖凝胶和卡波姆凝胶,辐照灭菌后观察其外观并检测其粘度、成模率、阻菌率、重金属含量、保湿率、水蒸气透过率、无菌性等理化特性和细胞毒性、皮内刺激、致敏性等生物相容性。取30只8~10周龄雄性SD大鼠,按照随机数字表法分为实验组、阳性对照组、阴性对照组,每组10只。在阴性对照组、阳性对照组、实验组大鼠背部制备全层皮肤缺损创面模型,分别进行常规换药、卡波姆凝胶换药、海藻糖凝胶换药处理。统计伤后6、12 d创面愈合率并记录创面愈合时间。伤后6 d,采用透射电镜观测大鼠创面组织中自噬体和自噬溶酶体数量,采用酶联免疫吸附测定法检测大鼠创面组织中微管相关蛋白轻链3Ⅰ(LC3Ⅰ)和LC3Ⅱ含量并计算其比值,采用天狼星红-苦味酸染色法检测大鼠创面组织中Ⅰ型胶原蛋白、Ⅲ型胶原蛋白占比及其比值和总胶原蛋白占比。前述实验样本数均为5。取3只3~4个月龄雄性新西兰白化模型兔,在每侧兔耳各制备3个深达软骨膜的创面,同前进行分组和处理,每组6个创面。创面愈合后30 d,大体观察并使用温哥华瘢痕量表评估兔耳瘢痕情况,采用苏木精-伊红染色检测兔耳瘢痕组织中表皮和真皮的厚度,采用天狼星红-苦味酸染色检测兔耳瘢痕组织中Ⅰ型胶原蛋白、Ⅲ型胶原蛋白占比及其比值和总胶原蛋白占比及其排布情况。样本数为6。结果辐照后的海藻糖凝胶和卡波姆凝胶均呈淡黄色透明状,无异味和杂质。海藻糖凝胶粘度、成膜率、阻菌率、保湿率均显著优于卡波姆凝胶(t值分别为4.13、3.50、4.03、5.80,P<0.05),但水蒸气透过率显著低于卡波姆凝胶(t=-4.14,P<0.05)。2种凝胶均未检出重金属和细菌。2种凝胶均无细胞毒性,皮内刺激和致敏性均为阴性�Objective To investigate the effects of trehalose gel on full-thickness skin defect wounds in rats and scar hyperplasia in rabbit ears.Methods The study was an experimental study.The trehalose gel and carbomer gel were prepared,their appearance after irradiation sterilization,and their physicochemical characterization such as viscosity,film forming rate,bacteria resistance rate,heavy metal content,moisture retention rate,water vapor permeability,sterility,and biocompatibility such as cytotoxicity,intradermal irritation,and sensitization were observed.Thirty male Sprague-Dawley rats aged 8-10 weeks were divided into experimental group,positive control group,and negative control group using a random number table method,with 10 rats in each group.The full-thickness skin defect wound models were prepared on the back of rats in negative control group,positive control group,and experimental group,and were treated with routine dressing change,carbomer gel dressing change,and trehalose gel dressing change,respectively.The wound healing rates on 6 and 12 days after injury and the wound healing time were recorded.On 6 days after injury,the number of autophagosomes and autophagolysosomes in rat wound tissue was detected using transmission electron microscopy.The content of microtubule associated protein light chain 3Ⅰ(LC3Ⅰ)and LC3Ⅱin rat wound tissue was detected using enzyme-linked immunosorbent assay method and their ratio was calculated.The proportion of typeⅠandⅢcollagens and their ratio,as well as the total collagen proportion in rat wound tissue were detected using sirius red picric acid staining method.The number of samples in the aforementioned experiments was all 5.Three male New Zealand albino model rabbits aged 3-4 months were taken,and 3 wounds deep to the perichondrium were created on each of the rabbit ears,with six wounds in each group and being grouped and treated as mentioned above.On 30 days after wound healing,the scar tissue of the rabbit ear was observed and evaluated using the Vancouver scar

关 键 词：生物相容性材料 海藻糖 瘢痕 自噬 创面修复 

分 类 号：R62[医药卫生—整形外科]