绵羊肝细粒棘球蚴包囊形成不同时期的病理学变化观察  

Pathological changes in the formation of Echinococcus granulosus cysts in sheep liver at different stages

作　　者：候梦丹 吉古孝安 刘伟伟 邱美龄 胡美荷 李昆雷 加依娜尔·吉克散巴依 翟少华[1] HOU Mengdan;JIGU Xiaoan;LIU Weiwei;QIU Meiling;HU Meihe;LI Kunlei;JIAYINAER Jikesanbayi;ZHAI Shaohua(College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830052,China;Secondary Vocational and Technical Schools in Hotan Prefecture,Hotan 848000,Xinjiang,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]和田地区中等职业技术学校,新疆和田848000

出　　处：《中国寄生虫学与寄生虫病杂志》2024年第3期316-324,共9页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：新疆维吾尔自治区自然科学基金重点项目(2023D01D04)。

摘　　要：目的 观察绵羊细粒棘球蚴包囊形成不同时期的肝细胞损伤、局部炎症反应,以及包囊纤维蛋白组成的病理学特征。方法 收集新疆乌鲁木齐市某屠宰场阿勒泰羊中有明显细粒棘球蚴包囊的肝脏,根据包囊的形态特征分为包囊发育期组、包囊形成期组和包囊成熟期组,取各组包囊与健康肝组织交界处的病变组织制备切片,以健康羊肝组织为对照组。苏木素-伊红(HE)染色观察包囊周围肝细胞形态特征及周围组织形态学变化,Masson染色观察包囊形成不同时期囊壁的纤维化过程,透射电子显微镜观察肝细胞超微结构变化,免疫组织化学染色观察不同时期包囊及其周围组织的炎症细胞CD3^(+)T淋巴细胞(CD3^(+))、CD25^(+)调节性T细胞(CD25^(+))、CD56^(+)自然杀伤细胞(CD56^(+))、CD14^(+)单核巨噬细胞(CD14^(+))、嗜碱粒细胞CD63 (CD63),以及纤维化蛋白Ⅰ型胶原蛋白1 (COL1)、COL3、α-平滑肌动蛋白(α-SMA)、钙结合蛋白A4 (S100A4)、基质金属蛋白酶2 (MMP2)和细胞因子肿瘤坏死因子-α (TNF-α)、血管内皮生长因子受体-3 (VEGFR-3)的表达变化。组间比较采用单因素ANOVA分析,两两比较采用LSD-t检验。结果 HE染色结果可见,包囊发育期,囊壁有明显的炎症反应带及炎症细胞团的形成,炎症细胞向囊外周肝实质扩散;包囊形成期,囊壁变薄,炎症细胞数量明显减少,炎症反应带变薄;包囊成熟期,囊壁纤维组织增生形成包囊的外壁角质层,炎症细胞数量减少。Masson染色结果可见,包囊发育期的囊壁外周有大量纤维组织产生,并向周围肝组织内延伸;包囊形成期包囊生长发育,炎症反应减弱,囊壁纤维组织发育成熟;包囊成熟期形成致密的纤维性囊壁。透射电子显微镜观察可见,随着包囊发育形成,肝细胞线粒体逐渐肿大、数量增多,肝细胞中的脂滴数量增多、体积增大。免疫组织化学染色结果显示,随着包囊的增大,囊壁炎症�Objective To observe the pathological characteristics of hepatocyte damage, local inflammation response, and the composition of cyst fibrin in liver of Echinococcus granulosus infected sheep during the cyst formation at different stages.Methods Livers with significant E. granulosus cysts were collected from slaughtered Altai sheep in an abattoir in Urumqi City, Xinjiang, China. From the sampled livers, cysts were separated and assigned into the groups of development stage, formation stage and mature stage according to the morphological features of the cysts.The cysts of all groups and the lesion tissues at the junction with healthy liver tissues were used to prepare paraffin sections. Liver tissues from healthy sheep were used as the control. The morphological features and changes of the hepatocytes and liver tissues around the cysts were observed by hematoxylin-eosin(HE) staining, and the fibrosis process of the cyst wall at different formation stages was observed by Masson staining. Transmission electron microscope was used to observe the ultrastructural changes of hepatocytes. Immunohistochemical staining was used to observe the cysts and surrounding tissues at different cyst-formation stages for the changes of inflammatory cells CD3^(+)T lymphocytes(CD3^(+)), CD25^(+)regulatory T cells(CD25^(+)), CD56^(+)NK cells(CD56^(+)), CD14^(+)monocyte macrophages(CD14^(+)) and basophilic granulocytes CD63(CD63), as well as the changes of expression of fibrotic proteins collagen type I(COL1),COL3, alpha-smooth muscle actin(a-SMA), calcium-binding protein A4(S100A4), matrix metalloproteinase 2(MMP2)and tumour necrosis factor-alpha(TNF-α), and vascular endothelial growth factor receptor-3(VEGFR-3). One-way ANOVA analysis was used for comparison between groups, and the LSD-t test was used for pairwise comparison.Results The HE staining results showed that the liver exhibited significant inflammatory reaction bands and inflammatory cell clusters in the cyst wall during the encapsulation development stage. The inflammatory c

关 键 词：细粒棘球蚴 包囊 病理学 炎性细胞 纤维化 绵羊 

分 类 号：R532.32[医药卫生—内科学]