普通烟草HSP20基因家族成员鉴定及表达分析  

Identification and expression analysis of HSP20 gene family members in common tobacco

作　　者：赵泽玉 杜甫 李子玮 王琰琰 王荣浩 施友志 孙晋浩 田震 赵清海[4] 管恩森[4] 孙刚[4] 王大海[4] 王朔 李志远 ZHAO Zeyu;DU Fu;LI Ziwei;WANG Yanyan;WANG Ronghao;SHI Youzhi;SUN Jinhao;TIAN Zhen;ZHAO Qinghai;GUAN Ensen;SUN Gang;WANG Dahai;WANG Shuo;LI Zhiyuan(China Tobacco Hubei Industrial LLC,Wuhan 430040,China;Institute of Tobacco Research of CAAS,Qingdao 266101,China;Technology Center of China Tobacco Jiangsu Industrial LLC,Nanjing 210011,China;Shandong Weifang Tobacco Co.,Ltd,Weifang 261031,China)

机构地区：[1]湖北中烟工业有限责任公司,湖北省武汉市430040 [2]中国农业科学院烟草研究所,山东省青岛市266101 [3]江苏中烟工业有限责任公司技术中心,江苏省南京市210019 [4]山东潍坊烟草有限公司,山东省潍坊市261031

出　　处：《中国烟草学报》2024年第4期74-83,共10页Acta Tabacaria Sinica

基　　金：中国烟草总公司重大科技项目“基于工业导向的云烟87多性状聚合育种研究”(110202201014(JY-14));中央级公益性科研院所基本科研业务费专项(1610232023013);江苏中烟科技项目“中烟特香301新品种烟叶品质保障技术及应用研究”(H202110)。

摘　　要：【背景和目的】热休克蛋白20(Heat Shock Protein 20,HSP20)是一个重要的蛋白质家族,在植物生长发育及抗逆胁迫中发挥重要作用,目前HSP20基因在烟草中还未有广泛的研究。【方法】通过茄科数据库,利用生物信息学分析手段,确定普通烟草中HSP20家族成员并进行相关分析。【结果】在烟草中鉴定得到了58个HSP20家族基因,进化分析将其划分为13个亚家族,分布在13条染色体上;共线性分析表明烟草中有5对基因存在染色体片段复制;顺式作用元件分析表明NtHSP20成员上游存在生长发育、胁迫响应等多种顺式作用元件;HSP20家族基因具有显著的组织特异表达性,在根、茎中基因表达数量和表达水平较明显,在花中的表达数量和表达水平较低;qRT-PCR分析表明NtHSP20家族部分成员对干旱胁迫具有特异性响应,且在不同的胁迫阶段不同基因表达量有显著差异。【结论】该研究为后续烟草HSP20家族生长发育和抗逆胁迫等机理研究奠定了基础。[Background]Heat Shock Protein 20(HSP20)is an important protein family that plays a critical role in plant growth,development,and stress resistance.Currently,there is limited comprehensive research on HSP20 genes in tobacco.[Methods]Using bioinformatics analysis tools and the Solanaceae database,this study identified members of the HSP20 family in common tobacco and conducted related analyses.[Results]Fifty-eight HSP20 family genes were identified in tobacco,and evolutionary analysis divided them into 13 subfamilies,distributed across 13 chromosomes.Collinearity analysis indicated that there were 5 pairs of genes with chromosome segment duplications in tobacco.Cis-acting element analysis showed that upstream of NtHSP20 members contained a variety of cis-acting elements related to growth and stress response.The HSP20 family genes exhibited significant tissue-specific expression,with higher gene expression quantity and level in roots and stems,and lower in flowers.qRT-PCR analysis indicated that some members of the NtHSP20 family have a specific response to drought stress,with significant differences in gene expression levels at different stress stages.[Conclusion]This study lays the foundation for further research into the mechanisms of growth,development,and stress resistance in the tobacco HSP20 family.

关 键 词：烟草 NtHSP20 进化分析 抗逆胁迫 表达分析 

分 类 号：S572[农业科学—烟草工业]