NtJAZ9基因参与调控烟草腺毛发生的功能研究  

Functional Study on the Role of NtJAZ9 Gene in Regulating the Formation of Tobacco Glandular Trichomes

作　　者：朱文奇 张新要 裴文毅 季盈彤 董千锋 廖子鑫 闫筱筱 崔红[1] ZHU Wenqi;ZHANG Xinyao;PEI Wenyi;JI Yingtong;DONG Qianfeng;LIAO Zixin;YAN Xiaoxiao;CUI Hong(College of Tobacco Science,Henan Agricultural University,Zhengzhou 450046,China;Technology Center of China Tobacco Hunan Industrial Corporation,Changsha 410007,China)

机构地区：[1]河南农业大学,烟草学院,郑州市450046 [2]湖南中烟工业有限责任公司技术中心,湖南省长沙市410007

出　　处：《中国烟草学报》2024年第4期95-104,共10页Acta Tabacaria Sinica

基　　金：国家自然科学基金青年基金“中国野生毛葡萄VqJAZ4抗白粉病功能及调控机制研究”(32102361);湖南中烟工业有限责任公司合作项目“多腺毛、高烟碱K326种质创制和育种应用研究—多腺毛高烟碱K326材料的创制和分析”(202143000834089)。

摘　　要：【目的】探究茉莉酮酸调控烟草腺毛发生的分子机制。【方法】对茉莉酮酸信号关键抑制子NtJAZ9进行同源克隆、生物信息学分析和基因功能研究。【结果】(1)NtJAZ9有两条同源序列NtJAZ9-1和NtJAZ9-2,编码区长度分别为1008 bp和996 bp。(2)亚细胞定位结果表明NtJAZ9蛋白定位在细胞核中。在各组织中NtJAZ9-1相对表达量均高于NtJAZ9-2,NtJAZ9-1在茎表皮中表达量最高,NtJAZ9-2在叶中表达量最高。(3)NtJAZ9过表达植株叶面腺毛密度降低,NtJAZ9基因敲除植株腺毛密度显著增加,保护毛密度差异不显著。茉莉酮酸甲酯(methyl jasmonate,MeJA)可显著促进长柄腺毛的发生,喷施后过表达株系长柄腺毛密度增加1.76倍,敲除株系长柄腺毛增加0.36倍。【结论】NtJAZ9基因参与调控烟草腺毛的发生,并在一定程度上影响了MeJA信号对烟草腺毛发生的调控作用。[Purpose]This study aims to explore the molecular mechanism of jasmonic acid in regulating tobacco trichomes glandular trichomes occurrence.[Methods]Homologous cloning,bioinformatics analysis and gene function studies of NtJAZ9,a key repressor of jasmonic acid signaling,were conducted[Results](1)NtJAZ9 has two homologous sequences,NtJAZ9-1 and NtJAZ9-2,with coding regions of 1008 bp and 996 bp,respectively.(2)Subcellular localization results indicate that the NtJAZ9 protein is localized in the nucleus.In various tissues,the relative expression of NtJAZ9-1 is higher than that of NtJAZ9-2,with the highest expression of NtJAZ9-1 in stem epidermis and the highest expression of NtJAZ9-2 in leaves.(3)Overexpression of NtJAZ9 results in reduced glandular trichome density on leaves,while knockout of the NtJAZ9 gene significantly increases glandular trichome density;the difference in protective trichome density is not significant.Methyl jasmonate(MeJA)can significantly promote the occurrence of long-stalk glandular trichomes.After spraying,the density of long-stalk glandular trichomes increased by 1.76 times in overexpressed strains and by 0.36 times in knockout strains.[Conclusion]The NtJAZ9 gene is involved in the regulation of occurrence of tobacco glandular trichomes and affects the regulatory role of MeJA signals on the formation of tobacco glandular trichomes to a certain extent.

关 键 词：烟草 NtJAZ9 过表达 基因编辑 腺毛密度 MEJA 

分 类 号：S572[农业科学—烟草工业]