基于RNA检测畜禽病原菌活菌方法研究进展  

Research progress of RNA-based detection of viable pathogens in livestock and poultry

作　　者：沈静怡 徐佳瑶 陈赵圆 刘晓明 庄林林 申秋平 Shen Jingyi;Xu Jiayao;Chen Zhaoyuan;Liu Xiaoming;Zhuang Linlin;Shen Qiuping(Jiangsu Vocational College of Agriculture and Forestry,Jiangsu Zhenjiang 212400)

机构地区：[1]江苏农林职业技术学院,江苏镇江212400

出　　处：《现代畜牧兽医》2024年第6期81-85,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：大学生创新创业培育计划项目(202313103017Y);江苏农林职业技术学院青年扶持项目(2022kj32);江苏农林职业技术学院亚夫科技与服务重点项目(2023kj08);江苏省高等学校基础科学(自然科学)研究面上项目(22KJB180001)。

摘　　要：致病菌对人类健康和畜牧业发展造成了巨大威胁,因此,快速精准检测具有感染能力的病原菌活菌具有重要的公共卫生学意义。已有研究表明,RNA分子与细胞的代谢活性高度相关,并且会在细菌死亡后迅速降解,因此RNA具有作为活菌分子标志物的应用价值。文章对已应用于常见畜禽病原菌活菌检测的RNA分子进行了分类和总结,并讨论了当前相关方法的优缺点和面临的挑战,最后展望了RNA的活菌检测方法未来的发展方向。期待未来能够发掘更多的新靶标并发展更多的新兴技术,为科学防控畜禽病原菌感染及相关传染病的临床诊断提供更多有力工具支持。Pathogenic bacteria pose a great threat to human health and the development of animal husbandry.Therefore,rapid and accurate detection of live pathogenic bacteria with infectious ability has important public health significance.Studies have shown that RNA molecules are highly correlated with the metabolic activity of cells,and will degrade rapidly after the death of bacteria,so RNA has the application value as a molecular marker of living bacteria.In this paper,the RNA molecules that have been used to detect live bacteria of common livestock and poultry pathogens are classified and summarized,and the advantages,disadvantages and challenges of current related methods are discussed.Finally,the future development direction of RNA-based live bacteria detection methods is prospected.It is expected that more new targets will be discovered and more emerging technologies will be developed in the future,which will provide more powerful tools for scientific prevention and control of livestock and poultry pathogenic bacteria infection and clinical diagnosis of related infectious diseases.

关 键 词：畜禽病原菌 活菌检测 RNA 检测方法 

分 类 号：S855.1[农业科学—临床兽医学]