不同产地手参药材质量评价:基于UPLC-Q-TOF-MS结合多元统计方法  

Comprehensive Assessment of the Quality of Gymnadenia Conopsea P.Br from Various Origins,Utilizing UPLC-Q-TOF-MS in Conjunction with a Multivariate Statistical Approach

作　　者：玉莲 代小明 姜山丹 包淑兰 萨日盖 陈玉兰[1] 包晓华[1] 德林 包黎明[1,2] Yu Lian;Dai Xiaoming;Jiang Shandan;Bao Shulan;Sa Rigai;Chen Yulan;Bao Xiaohua;De Lin;Bao Liming(College of Mongolian Medicine and Pharmacy,Inner Mongolia Minzu University,Tongliao 028000,China;Key Laboratory of Mongolian Medicine Research and Development Engineering,Ministry of Education,Inner Mongolia Minzu University,Tongliao 028000,China;Tongliao Traditional Chinese Medicine Industry Development Office,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学蒙医药研发工程教育部重点实验室,内蒙古通辽028000 [3]内蒙古通辽市中药产业发展办公室,内蒙古通辽028000

出　　处：《亚太传统医药》2024年第7期72-77,共6页Asia-Pacific Traditional Medicine

基　　金：内蒙古自治区重点研发和成果转化计划项目(2023YFDZ0063);内蒙古自治区直属高校青年教师提升科研创新能力计划项目(GXKY22139);内蒙古自治区留学回区人员创新启动支持计划项目(2022LXCX002)。

摘　　要：目的:比较不同产地手参化学成分,为判别不同产地手参及质量评价提供依据。方法:采用超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS),对于不同来源的手参进行化学成分比较。利用主成分分析法(PCA)和正交偏最小二乘法判别分析法(OPLS-DA)对4种产地24批药材进行多元统计分析,并筛选其潜在的候选判别化合物。结果:经多元统计分析结果显示,不同产地的化学成分差异显著,共鉴定出21个差异显著的化学成分。其中,化合物1、5、8、10、11、13、17、18在安徽亳州手参中的含量最高;化合物3、6、7、9、14、16及21在四川手参中含量最高。化合物2、12及20在黑龙江手参中的含量较高。在内蒙古手参里仅有化合物4的含量相对较高。结论:不同产地手参所含化学成分差异显著,可通过这些差异化合物作为指标性成分区分不同产地。此方法为手参的质量控制及进一步开发利用提供依据。Objective:To analyze the chemical constituents of Gymnadenia Conopsea P.Br from various production areas and provide a foundation for the identification and quality assessment of Gymnadenia Conopsea P.Br from different origins.Methods:Ultra performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS)for analyzing the components of Gymnadenia Conopsea P.Br from diverse locations.Principal component analysis(PCA)and Orthogonal partial least squares discriminant analysis(OPLS-DA)were utilized for multivariate statistical analysis on 24 batches of medicinal materials from four origins,thereby screening potential candidate discriminant compounds.Results:The results showed that the chemical compositions of different origins differed significantly from each other,and a total of 21 chemical compounds with significant differences were identified.Among them,compounds 1,5,8,10,11,13,17 and 18 were found in Bozhou,Anhui Province,with the highest content;compounds 3,6,7,9,10,11,13,17 and 18 were found in Bozhou,Anhui Province,with the highest content.Compounds 3,6,7,9,14,16 and 21 were the most abundant in Sichuan Gymnadenia conopsea P.Br.Compounds 3,6,7,9,14,16 and 21 were the most abundant in Sichuan Ginseng.The contents of compounds 2,12 and 20 were higher in Heilongjiang Gymnadenia conopsea P.Br.Only compound 4 was found in Inner Mongolian Gymnadenia conopsea P.Br.The content of compound 4 was relatively high in Inner Mongolian Gymnadenia conopsea P.Br.Conclusion:The differences in chemical composition of ginseng from different places of origin are significant,and these different compounds can be used as index components to distinguish different places of origin.This method provides a basis for the quality control and further development and utilization of ginseng.

关 键 词：手参 超高压液相色谱飞行时间质谱仪 化学成分 质量评价 

分 类 号：R284[医药卫生—中药学]