HPLC法同时测定苏杏痰咳合剂中苦杏仁苷和柚皮苷的含量  

Determination of Amygdalin and Naringin in Suxing Tanke Mixture by HPLC

作　　者：陈钰婷 何燕莉 温颖婉 叶淑芳 Chen Yuting;He Yanli;Wen Yingwan;Ye Shufang(Guangzhou Panyu District Institute for Food and Drug Control,Guangzhou 511400,China;Guangzhou Hospital of Integrated Traditional and West Medicine,Guangzhou 510801,China)

机构地区：[1]广州市番禺区食品药品检验所,广东广州511400 [2]广州市中西医结合医院,广东广州510801

出　　处：《亚太传统医药》2024年第7期77-80,共4页Asia-Pacific Traditional Medicine

基　　金：广东省“十三五”中医重点专科(临床药学)建设项目(粤中医函[2019]472号);广州市中医药和中西医结合科技项目(20232A011026);广州市花都区医疗卫生一般科研专项项目(23-HDWS-043)。

摘　　要：目的:建立高效液相色谱法同时测定医院制剂苏杏痰咳合剂中苦杏仁苷和柚皮苷的含量。方法:采用Phenomenex C_(18)色谱柱(4.6 mm×250 mm,5μm)分离,流动相为0.1%磷酸溶液-乙腈(80∶20),流速1.0 mL/min,柱温30℃,检测波长207 nm和283 mm。结果:苦杏仁苷和柚皮苷在5~150μg/mL范围内线性关系良好,平均回收率分别为102.2%和96.3%,精密度、稳定性、重复性试验的RSD均小于2.1%。结论:该方法简便、准确、重现性好,为进一步提升苏杏痰咳合剂的质量控制提供科学参考。Objective:To establish an HPLC method for the simultaneous determination of amygdalin and naringin in the hospital preparation Suxing Tanke Mixture.Methods:The chromatography was separated on Phenomenex C_(18)column(4.6 mm×250 mm,5μm)by using elution with 0.1%phosphoric acid solution-acetonitrile(80∶20)as mobile phase at the flow rate of 1.0 mL/min.The column temperature was 30℃.The detection wavelengths were 207 nm and 283 mm.Results:Amygdalin and naringin had a good linear relationship in the range of 5~150μg/mL The average recoveries were 102.2%and 96.3%,respectively.RSD of precision,stability and reproducibility tests were lower than 2.1%.Conclusion:The method is simple,accurate and reproducible,which can provide scientific reference for further improving the quality control of Suxing Tanke Mixture.

关 键 词：苏杏痰咳合剂 质量标准 高效液相色谱 苦杏仁苷 柚皮苷 

分 类 号：R284.2[医药卫生—中药学]