假禾谷镰孢毒素化学型快速鉴定及在分析黄淮麦区分离物中的应用  

A rapid method for chemotype identification of Fusarium pseudograminearum and its application on the analysis of the isolates from Huanghuai wheat-growing region of China

作　　者：邓渊钰[1,2] 刘振国 陈琛 孙海燕 曹淑琳[1,2] 李伟 陈怀谷 DENG Yuanyu;LIU Zhenguo;CHEN Chen;SUN Haiyan;CAO Shulin;LI Wei;CHEN Huaigu(Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014;Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009;School of Life Sciences,Westlake University,Hangzhou 310024)

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014 [2]江苏省粮食作物现代产业技术协同创新中心,扬州225009 [3]西湖大学生命科学学院,杭州310024

出　　处：《植物病理学报》2024年第3期481-492,共12页Acta Phytopathologica Sinica

基　　金：国家现代农业产业技术体系项目(CARS-03)。

摘　　要：假禾谷镰孢(Fusarium pseudograminearum)是我国小麦茎基腐病的优势病原菌之一,为了快速简便地鉴定其毒素化学型,本研究检验了4种已报道的镰孢菌毒素化学型分子生物学鉴定方法在该菌上的适用性,结果显示这些方法均不能有效区分它的三种化学型。基于此,本研究设计特异性引物,并建立了能用于F.pseudograminearum毒素化学型分子生物学鉴定的方法。采用此方法,对从河北、山东、河南和江苏采集的479株F.pseudograminearum的化学型进行了鉴定,发现这些菌株中只有极少数为NIV型,其余均为DON型。河北、山东和河南的F.pseudograminearum以15-ADON化学型为主,而江苏的菌株中3-ADON化学型则占多数。F.pseudograminearum的3-ADON和15-ADON化学型的地理分布与禾谷镰孢复合种相似。Fusarium pseudograminearum is one of the dominant pathogens causing wheat crown rot in China.To identify its chemotypes quickly and simply,the 4 reported methods for Fusarium chemotype identification were tested for their applicability to this pathogen.The results showed that these methods could not effectively differentiate the three chemotypes of F.pseudograminearum.In this case,we explored a new method to identify the chemotypes of F.pseudograminearum by designing specific primers.Using this new developed method,the chemotypes of 479 strains of F.pseudograminearum collected from Hebei,Shandong,Henan and Jiangsu provinces,China were identified.Besides rare NIV producers,theses isolates are mainly DON producers.Most isolates from Hebei,Shandong and Henan provinces belong to 15-ADON in chemotype,but the isolates from Jiangsu Province primarily belong to the chemotype of 3-ADON.The geographic distribution of the 3-ADON and 15-ADON isolates of F.pseudograminearum is similar to that of F.graminearum species complex.

关 键 词：小麦茎基腐病 假禾谷镰孢 毒素化学型 特异性引物 3-ADON 15-ADON NIV 

分 类 号：S432.1[农业科学—植物病理学]