蝴蝶兰不同部位CymMV和ORSV同步检测及含量比较研究  

Simultaneous detection and comparison of cymbidium mosaic virus and odontoglossum ringspot virus levels in different parts of Phalaenopsis Aphrodite

作　　者：孙爱青 王丽花[2] 张艺萍[2] 杨秀梅[2] 苏艳[2] 吴学尉[1] SUN Aiqing;WANG Lihua;ZHANG Yiping;YANG Xiumei;SU Yan;WU Xuewei(Yunnan University,Kunming 650091,China;Flower Research Institute,Yunnan Academy of Agriculture Science/Yunnan Key Laboratory for Flower Breeding/National Engineering Research Center for Ornamental Horticulture,Kunming 650205,China)

机构地区：[1]云南大学,昆明650091 [2]云南省农业科学院花卉研究所/云南省花卉育种重点实验室/国家观赏园艺工程技术研究中心,昆明650205

出　　处：《植物病理学报》2024年第3期504-511,共8页Acta Phytopathologica Sinica

基　　金：云南省种子种业联合实验室项目(202205AR070001-05);云南省标准化研究项目(2023BZHXM05);国家花卉产业体系昆明综合试验站项目(CARS-23-G56)。

摘　　要：为探究建兰花叶病毒(cymbidium mosaic virus,CymMV)和齿兰环斑病毒(odontoglossum ringspot virus,ORSV)在蝴蝶兰不同部位的RNA质量及带毒量,以携带2种病毒的植株为试验材料,采用基于TaqMan探针双重实时荧光定量PCR法检测蝴蝶兰的蕊柱、唇瓣、萼片、花梗(韧皮部)、叶片、气生根和地下根7个部位,并采用基因芯片法对带毒量进行复检验证。结果表明,蝴蝶兰不同部位的RNA提取效果存在差异,蕊柱的浓度最高,其次是唇瓣和气生根,地下根的浓度最低。双重实时荧光定量PCR检测结果表明,CymMV和ORSV在唇瓣和气生根中载量最高,在蕊柱和花梗中载量最低,2种病毒在不同部位的病毒载量变化趋势一致。本研究为蝴蝶兰病毒监测样本采集的部位选择提供了一定的理论指导。In order to investigate the RNA quality and virus content of CymMV and ORSV in different parts of Phalaenopsis aphrodite,plants infected with two viruses were used as experimental materials,a duplex TaqMan fluorescence quantitative PCR method was used to detect 7 sites of P.aphrodite:gynostemium,labellum,sepal,peduncle,leaf,aerial root and subterranean root.And the gene chip was used as a comparison method.The results showed that the RNA extraction effect of different parts was different.The RNA concentration of gynostemium was the highest,followed by labellum and aerial,and the lowest concentration was subterranean root.The results of duplex TaqMan fluorescence quantitative PCR showed that the CymMV and ORSV contents were the highest in the labellum and aerial root,and the lowest in the gynostemium and peduncle.The levels of both viruses also showed the same trend.This study provides some theoretical guidance for the selection of explants of P.aphrodite devirus seedlings and the selection of sites for virus monitoring samples.

关 键 词：蝴蝶兰 CymMV ORSV 检测带毒率 不同部位比较 

分 类 号：S432.44[农业科学—植物病理学]