基于NP基因序列的云南烟草番茄环纹斑点病毒群体遗传多样性分析  

Population genetic diversity analysis of tomato zonate spot virus on tobacco in Yunnan based on NP gene sequence

作　　者：赵正婷 盖晓彤 张俊蕾 夏振远[1] 马俊红[1] 刘雅婷 姜宁 ZHAO Zhengting;GAI Xiaotong;ZHANG Junlei;XIA Zhenyuan;MA Junhong;LIU Yating;JIANG Ning(Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650021,China;College of Agronomy and biotechnology,Yunnan Agriculture University,Kunming 650201,China;College of Plant Protection,Yunnan Agriculture University,Kunming 650201,China;College of Tobacco Science,Yunnan Agriculture University,Kunming 650201,China)

机构地区：[1]云南省烟草农业科学研究院,昆明650021 [2]云南农业大学农学与生物技术学院,昆明650201 [3]云南农业大学植物保护学院,昆明650201 [4]云南农业大学烟草学院,昆明650201

出　　处：《植物病理学报》2024年第3期512-521,共10页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(32260681);云南省烟草公司科技计划项目(2021530000242030,2021530000242031);云南省科技厅基础研究专项(202101AU070129)。

摘　　要：为探明云南烟草上番茄环纹斑点病毒(tomato zonate spot virus,TZSV)的发病情况及群体遗传多样性特征,本研究使用核壳体蛋白(nucleocapsid protein,NP)基因的特异性引物对采自云南各烟区的560份烟草斑萎病样品进行检测,对部分阳性样本的NP基因进行克隆测序,并对获得的序列进行系统发育、遗传变异、群体多样性等分析。RT-PCR结果显示,560份样品中共有262个检出TZSV,平均阳性检出率为46.8%;重组分析在DX_LJHP_65分离物基因序列中检测到显著的重组信号;系统发育分析显示129个TZSV分离物在进化上可以分为6个组,相同地区来源的分离物倾向于相聚成簇,表明该病毒在进化上具有较强的地理特异性;错配分布分析结果,云南TZSV群体呈多峰分布,表明该群体近期未经历群体扩张事件;种群间遗传分化分析结果表明,滇西和滇东北与其他群体之间基因交流频率很低,其余种群间存在频繁的基因交流。本研究是对云南各烟区TZSV分离物群体遗传变异情况的首次报道,研究结果可为防控TZSV提供重要参考。To investigate the incidence and population genetic diversity of tomato zonate spot virus(TZSV)on tobacco in Yunnan,560 samples of tobacco plants exhibiting symptoms of spot wilt were collected from various tobacco regions in this province.A pair of specific primers from the nucleocapsid protein(NP)gene were used to screen the samples.NP gene of the selected TZSV-positive samples was cloned,sequenced,and employed for phylogenetic,analyses,assessment of genetic diversity and determination of population differentiation.The results of RT-PCR showed that 262 out of the 560 samples were found to be infected with TZSV,with a mean positive detection rate of 46.8%.A significant recombination signal within the genome of the DX_LJHP_65 isolate was identified through recombination analysis.Phylogenetic analysis showed that 129 TZSV isolates can be divided into 6 groups,which tended to cluster according to their geographical origin,suggesting that the evolution of TZSV exhibits a pronounced geographical specificity.The mismatch distribution analysis results for the TZSV population in Yunnan indicated a multimodal distribution,suggesting that it has not undergone recent demographic expansion.The analysis of genetic differentiation among populations revealed a significant difference in gene exchange patterns.Specifically,the frequency of gene flow between the DX and DDB populations and other populations is found to be very low.On the other hand,there was frequent gene exchange observed between the remaining populations.This study is the first report on the genetic variation of TZSV isolate populations in various tobacco regions of Yunnan.It offers crucial insights that serve as a valuable reference for the prevention and control of TZSV.

关 键 词：烟草 番茄环纹斑点病毒 NP基因 遗传变异 遗传多样性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]